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Method and means for parallel frequency acquisition in frequency domain fluorometry

  • US 5,257,202 A
  • Filed: 11/27/1990
  • Issued: 10/26/1993
  • Est. Priority Date: 02/13/1989
  • Status: Expired due to Term
First Claim
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1. A digital frequency domain system for measuring the light emitted by a sample when excited by a light source having harmonic content, said system comprising:

  • (a) means for exciting said sample to emit a light signal, said means including a light source having a harmonic content and a predetermined fundamental frequency thereof;

    (b) a first means for detecting the light emitted by the sample to generate a first electrical signal containing information representative of the response of said sample at said fundamental and harmonic frequencies;

    (c) a second means for generating a second electrical signal representative of said fundamental frequency and a predetermined correlation frequency;

    (d) cross-correlation means for mixing said first and second electrical signals to generate a third electrical signal having a response at the fundamental and harmonic frequencies imposed on said correlation frequency;

    (e) means for digitizing said third electrical signal to obtain a plurality of data values for each period of the correlation frequency;

    (f) a data processing means for simultaneously calculating from said plurality of data values the phase and modulation values of the response of the sample at the fundamental and a predetermined number of said harmonic frequencies.

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