Continuous process for ethanol production from lignocellulosic materials without mechanical agitation

• US 5,258,293 A
• Filed: 05/03/1991
• Issued: 11/02/1993
• Est. Priority Date: 05/03/1991
• Status: Expired due to Term

• Current Assignee: The Trustees of Dartmounth College (Dartmouth College)
• Original Assignee: The Trustees of Dartmounth College (Dartmouth College)
• Inventors: Hogsett, David A., Spieles, Gisbert, Lynd, Lee R.
• Primary Examiner(s): Knode, Marian C.
• Application Number:
  US07/695,516
• Time in Patent Office: 914 Days
• Field of Search: 435/162, 435/161, 435/163, 435/165, 435/813, 426/14
• US Class Current: 435/165
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/813 Continuous fermentation

Abstract

An improved and highly productive method of continuously producing ethanol from lignocellulosic substrates is provided. The method involves providing a suitable microbial system within a reaction vessel and adding fermentable substrate to the reactor to form a reaction mixture. The fermentation reaction is allowed to proceed while a quiescent state is maintained within the reactor. During the fermentation, soluble substrate is differentially retained relative to the feed slurry and reaction biocatalysts are retained and internally recycled within the system. Further, while fermenting substrate is retained within the system, it forms a stratified zone within the reactor such that the concentration of actively fermenting substrate is highest at upper portions of the reaction zone and is lowest, near zero, at a lower portion of the reaction zone. Insoluble, fully reacted substrate may be withdrawn from a region near the bottom of the reactor.

Claims

1. A continuous process of producing ethanol from a lignocellulosic substrate, comprising the steps of:

providing a viable, aqueous culture of microorganisms without a reactor vessel;

continuously adding to the reactor vessel a feed slurry of fermentable, lignocellulosic substrate to form a reaction mixture;

allowing the fermentation reaction to proceed while maintaining a substantially quiescent state without mechanical agitation within the reactor vessel, resulting in the formation within the reactor vessel of at least an upper, gaseous zone, an intermediate, clarified liquid zone and a lower, substantially stratified reaction bed zone in which the concentration of fermentable substrate is higher at the top of the reaction bed zone than at the bottom of the reaction bed zone;

differentially retaining fermentable substrate and adsorbed biocatalyst within the reactor vessel relative to the feed slurry during the fermentation reaction;

continuously withdrawing liquid from the intermediate zone; and

extracting the ethanol product from the liquid.

2. The process of claim 1, further comprising the steps of:

accumulating insoluble, reacted substrate components at a bottom portion of the reactor vessel; and

continuously withdrawing from the reactor vessel a slurry rich in the insoluble, reacted substrate components.

3. The process of claim 2 further comprising the steps of

separating the withdrawn slurry into a solid substrate component and an aqueous component;

and

combining the aqueous component with the withdrawn liquid from the intermediate zone.

4. The process of claim 1 wherein the fermentation is conducted by Direct Microbial Conversion.

5. The process of claim 4 wherein the culture of microorganisms is selected from the group consisting of Clostridium thermocellum, Fusarium oxysporum, and Clostridium cellulolyticum.

7. The process of claim 1 wherein the fermentation is conducted by Simultaneous Saccharification and Fermentation.

8. The process of claim 7 wherein the culture of microorganisms is selected from the group consisting of Trichoderma reesei, Saccharomyces cerevisiae, Acidothermus cellulolyticus, Trichoderma koningii, and Zymomonas mobilis.

9. The method of claim 1 wherein the lignocellulosic substrate is wood particles.

10. The process of claim 1 wherein the lignocellulosic substrate comprises particles of mixed hardwood.

11. The process of claim 1 wherein the lignocellulosic substrate is plant matter.

12. The process of claim 1 wherein the lignocellulsic substrate is grasses.

13. The process of claim 1 wherein the lignocellulosic substrate is solid waste particles.

6. The process of claim 15 wherein the culture of microorganisms is in co-culture with pentose-utilizing organisms.