Autoradiographic process

• US 5,260,190 A
• Filed: 07/06/1989
• Issued: 11/09/1993
• Est. Priority Date: 11/05/1982
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Kato, Hisatoyo, Shiraishi, Hisashi, Miyahara, Junji
• Primary Examiner(s): Lovering, Richard D.
• Assistant Examiner(s): Covert, John M.
• Application Number:
  US07/378,509
• Time in Patent Office: 1,587 Days
• Field of Search: 424/1.1, 250/327.1, 250/303, 250/362, 250/361 C, 250/327.2, 250/484.1, 436/504, 436/542, 436/57, 436/94, 935/77, 435/6
• US Class Current: 435/6.11
• CPC Class Codes: G01T 1/2012 using stimulable phosphors, e.g. stimulable phosphor sheets
• CPC Class Codes: G01T 1/2014 Reading out of stimulable sheets, e.g. latent image
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

An autoradiographic process for obtaining information on one or two dimensional location of radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances such as DNA originating from an organism,

which comprises:

placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers for a certain period to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;

scanning said phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet as stimulated emission; and

detecting the stimulated emission to obtain locational information on the radioactively labeled substances in the sample.

Claims

1. An autoradiographic process for obtaining locational information on radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances originating from an organism,

which comprises:

placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in a layer at 10°

-35°

C. for a certain period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;

scanning said phosphor sheet with visible light or infrared rays to release at least a portion of said radiation energy stored in said phosphor sheet as stimulated emission; and

detecting the stimulated emission to obtain one or two dimensional information on the location of radioactively labeled substances in the sample.

2. The autoradiographic process as claimed in claim 1, wherein the sample containing radioactively labeled substances is a medium containing radioactively labeled tissue of an organism and/or a radioactively labeled substances originating from an organism.

3. The autoradiographic process as claimed in claim 2, wherein the medium is a support having radioactively labeled substances resolved thereon and said radioactively labeled substances are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element.

4. The autoradiographic process as claimed in claim 3, wherein said support is one which carries the radioactively labeled biopolymers electrophoretically resolved thereon.

5. The autoradiographic process as claimed in claim 4, wherein said biopolymers are nucleic acids, derivatives thereof, or cleavage products thereof.

6. The autoradiographic process as claimed in claim 5, which comprises steps of:

placing a support carrying a mixture of DNA fragments or DNA cleavage products electrophoretically resolved thereon, the mixture being labeled with a radioactive element, and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers for a certain period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactive element attached to the mixture of DNA fragments or DNA cleavage products,

scanning said phosphor sheet with visible light or infrared rays to release at least a portion of radiation energy stored in said phosphor sheet as stimulated emission; and

detecting the stimulated emission to obtain locational information on the DNA fragments or the DNA cleavage products on the support.

7. The autoradiographic process as claimed in any one of claims 1 to 6, wherein said stimulable phosphor sheet comprises a substrate, a phosphor layer comprising a stimulable phosphor dispersed on a binder, and a protective film.

8. A process for obtaining locational information on radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances originating from an organism,

which comprises:

placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers at 10°

-35°

C. for a certain period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;

scanning said phosphor sheet with visible light or infrared rays to release at least a portion of said radiation energy stored in said phosphor sheet as stimulated emission; and

detecting the stimulated emission to obtain one or two dimensional information on the location of radioactively labeled substances in the sample;

and

representing the locational information in the form of a visible image.

9. A process for obtaining locational information on radioactively labeled substances contained in a sample selected from the group consisting of tissue of an organism and a medium containing tissue of an organism and/or substances originating from an organism,

which comprises:

placing said sample and a stimulable phosphor sheet having a phosphor layer comprising a stimulable phosphor dispersed in a binder together in layers at 10°

-35°

for a certain period of time to cause said phosphor sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances in said sample;

scanning said phosphor sheet with visible light or infrared rays to release at least a portion of said radiation energy stored in said phosphor sheet as stimulated emission;

detecting the stimulated emission to obtain one or two dimensional information on the location of radioactively labeled substances in the sample; and

representing the locational information in the form of symbols, numerals, or a combination thereof.