Redox polymer modified electrode for the electrochemical regeneration of coenzyme
First Claim
1. A redox polymer-modified electrode for use in liquid mixtures of components for detecting the presence of, or measuring the amount of, one or more selected components by the electrochemical regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogues thereof, characterized by having an adsorbed redox polymer comprising polysiloxane, polyalkane, poly(ethylene oxide), poly(ethylene imine), or copolymers thereof, which contain covalently attached mediators comprising phenazinium ions, phenazinones, phenoxazinium ions, phenoxazones, phenothiazinium ions, or phenothiazinones, substituted or unsubstituted;
- an electrode substrate with said polymer on a surface thereof;
one or more enzymes, at least one of which is a de-hydrogenase, in dissolved, suspended or immobilized form on or at the electrode surface; and
NADH or NADPH coenzymes or analogues thereof dissolved or immobilized in said redox polymer, whereby said coenzyme can be re-oxidized by the electrode.
9 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to an electrochemical enzyme biosensor for use in liquid mixtures of components for detecting the presence of, or measuring the amount of, one or more selected components. The enzyme electrode of the present invention includes a redox polymer immobilized on an electrode surface, one or more enzymes, at least one of which is a de-hydrogenase, a coenzyme and an electron collector.
-
Citations
9 Claims
-
1. A redox polymer-modified electrode for use in liquid mixtures of components for detecting the presence of, or measuring the amount of, one or more selected components by the electrochemical regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogues thereof, characterized by having an adsorbed redox polymer comprising polysiloxane, polyalkane, poly(ethylene oxide), poly(ethylene imine), or copolymers thereof, which contain covalently attached mediators comprising phenazinium ions, phenazinones, phenoxazinium ions, phenoxazones, phenothiazinium ions, or phenothiazinones, substituted or unsubstituted;
- an electrode substrate with said polymer on a surface thereof;
one or more enzymes, at least one of which is a de-hydrogenase, in dissolved, suspended or immobilized form on or at the electrode surface; and
NADH or NADPH coenzymes or analogues thereof dissolved or immobilized in said redox polymer, whereby said coenzyme can be re-oxidized by the electrode. - View Dependent Claims (2, 3, 4, 5, 8, 9)
- an electrode substrate with said polymer on a surface thereof;
-
6. A method of making an electrode for the electrochemical regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogues thereof, comprising the steps of:
- preparing an electrode substrate;
depositing a redox polymer film and coenzymes of claim 10 onto said electrode surface from a common organic, aqueous or mixed aqueous-organic solution containing in a dissolved or suspended form redox mediator and said coenzyme;
depositing onto said redox polymer film from an aqueous or mixed aqueous-organic solvent a layer of enzymes, at least one of which is a de-hydrogenase, to allow the enzymes to penetrate said redox polymer film. - View Dependent Claims (7)
- preparing an electrode substrate;
Specification