Device and a method for separating liquid samples

• US 5,264,184 A
• Filed: 03/19/1991
• Issued: 11/23/1993
• Est. Priority Date: 03/19/1991
• Status: Expired due to Term

• Current Assignee: Minnesota Mining and Manufacturing Co (3M Company), Qiagen GmbH (Qiagen NV)
• Original Assignee: Minnesota Mining and Manufacturing Co (3M Company)
• Inventors: Aysta, James E., Colpan, Metin
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Trembley, T. A.
• Application Number:
  US07/671,448
• Time in Patent Office: 980 Days
• Field of Search: 422/101, 210/473, 210/474, 210/477, 210/482, 210/472
• US Class Current: 422/527
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A device (10) for separating liquid samples has a sample container (12), bottom wall (20) of which has an outlet opening (22) provided therein. The outlet opening (22) is joined by an outlet spout (24) extending in the axial direction of the sample container (12). On bottom wall (20) there is a separation layer (28). Below sample container (12), a collecting container (32) is arranged abutting sample container (12) such that an exchange of air between the interior of the collecting chamber (32) and the environment is possible, yet an escape of liquid is largely inhibited. The contact surface between collecting chamber (32) and sample container (12) and end (26) of the outlet spout (24) through which the liquid is discharged, are axially spaced apart.

Claims

1. A device for separating a liquid sample, comprising

a sample container (12) having an inlet opening (16) and an outlet opening (22) between which a separation layer (28) is arranged, said sample container having a bottom wall (20) in which said outlet opening (22) is located,

a collecting container (32), for liquid emerging through said outlet opening (22), having a tubular wall (37) and a rim (38) which defines an opening (36) therein, and

an outlet spout (24) joining and enclosing said outlet opening (22), said sample container (12) having a unperforated collar (40) formed thereon that is a barrier for, and coaxially surrounds, said outlet spout (24), unperforated collar (40) being shorter than said outlet spout (24), said a perforated collar (40) being located within collecting container (32), and

wherein said collecting container (32) abuts at least one of said sample container (12) and said outlet spout (24) adjacent to said rim (38), without providing an airtight sealing, and

an end of said outlet spout (24) extending through said opening (36) of said collecting container (32) and into the same.

2. The device of claim 1, wherein said collecting container (32) abuts said bottom wall (20) adjacent said rim (38).

3. The device of claim 1 wherein said collecting container (32) is loosely connected onto said unperforated collar by its opening (36) to provide a non-airtight seal.

4. The device of claim 3, wherein said unperforated collar (40) is about one-half the length of said outlet spout (24).

5. The device of claim 3, wherein said sample container (12), said outlet spout (24) and said unperforated collar (40) are formed integrally.

6. The device of claim 1, further comprising a channel (39) which is provided in said tubular wall (37) of said collecting container (32).

7. The device of claim 1, further comprising a recess (39) in the rim (38) of said collecting container (32) abutting said sample container (12).

8. The device of claim 1, wherein said abutting portion of collecting container (32) is provided with a ring of porous material (47) permeable to air which prevents the escape of liquid and aerosols.

9. The device of claim 1, wherein said collecting container (32) is plugged onto said outlet spout (24) at its opening (36).

10. The device of claim 1, wherein said outlet spout (24) tapers conically towards its end.

11. The device of claim 1, wherein said sample container (12) is substantially tubular in shape.

12. The device of claim 1, wherein said separation layer (28) is retained in abutment on said bottom wall (20) of said sample container (12) by a retaining ring (30), said retaining ring (30) being radially supported on the inner sides of said sample container (12).

13. The device of claim 1 comprising a plurality of sample containers (12), each having an outlet spout (24) with an end (26), and a plurality of collecting containers (32), each of said sample containers (12) having a respective collecting container (32) associated therewith, said sample containers being arranged side by side.

14. The device of claim 13 wherein said plurality of sample containers (12) has a common circumferential flange (72).

15. The device of claim 13 further comprising a chamber (66) which is subjected to a partial vacuum, into which said plurality of sample containers and said plurality of collecting containers (32) are inserted.

16. The device of claim 1 comprising a plurality of sample containers (12), each of said plurality of sample containers (12) being associated with a respective collecting container (32), which are provided in rows and columns, the columns being orthogonal relative to said rows, and wherein said plurality of sample containers are interconnected.

17. The device of claim 1 for use wherein said liquid sample comprises components selected from the class consisting of nucleic acids or proteins of plant, animal or human cells and wherein said separation layer comprises a material for selectively absorbing at least one of said components in said liquid sample.

18. The device of claim 17, wherein said selectively absorbing material is a layer of particles and said layer of particles is enclosed between two fixation means.

19. The device of claim 17, wherein said selectively adsorbing material is at least one of silica gell, dextran, agarose, or a derivative thereof.

20. The device of claim 1 wherein said separation layer (28) comprises a membrane having sorptive properties.

21. A method for the separation or extraction of bio-polymers, comprising the steps of

providing a device according to claim 1 and pressing or suctioning a liquid sample containing at least one bio-polymer through said separation layer (28) to effect a separation of said liquid sample.

22. The method according to claim 21 wherein said at least one bio-polymer is a nucleic acid or protein of plant, animal, or human cells.

23. The device according to claim 1 further comprising additional separation layers (28).

24. The device according to claim 1 wherein said separation layer comprises a fibrillated polytetrafluoroethylene matrix having sorptive particulate emeshed therein.