Mammalian expression vector

• US 5,266,490 A
• Filed: 03/28/1991
• Issued: 11/30/1993
• Est. Priority Date: 03/28/1991
• Status: Expired due to Term

• Current Assignee: Regeneron Pharmaceuticals Incorporated
• Original Assignee: Regeneron Pharmaceuticals Incorporated
• Inventors: Davis, Samuel, Yancopoulos, George D.
• Primary Examiner(s): Schwartz, Richard A.
• Assistant Examiner(s): CARTER, PHILIP
• Application Number:
  US07/678,408
• Time in Patent Office: 978 Days
• Field of Search: 435/320.1, 435/69.1, 435/172.3, 536/27, 536/24.1, 536/23.4, 536/23.5
• US Class Current: 435/320.1
• CPC Class Codes: C07K 14/48 Nerve growth factor [NGF]
• CPC Class Codes: C07K 14/71 for growth factors; for growth regulators
• CPC Class Codes: C07K 14/82 Translation products from oncogenes
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/41 containing a Myc-tag
• CPC Class Codes: C07K 2319/61 containing an enzyme fusion for detection (lacZ, luciferase)
• CPC Class Codes: C07K 2319/75 containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2800/108 episomal vectors

Abstract

DNA plasmid expression vector, pCMX, enables cDNA expression cloning in mammalian cell culture. This novel expression vector exhibits a marked increase in gene expression when compared to its parental plasmid construction.

Claims

1. The DNA vector pCMX as deposited with the NRRL, having accession number NRRL B-18790.

2. The DNA vector of claim 1 further comprising a structural gene inserted in the 5'"'"'→

3'"'"' orientation within the polylinker sequence.

3. The DNA vector of claim 1 further comprising a structural gene inserted in the 3'"'"'→

5'"'"' orientation within the polylinker sequence.

4. The DNA vector of claim 1 further comprising a structural gene which encodes NGF-myc inserted in the 5'"'"'→

3'"'"' orientation within the polylinker sequence.

5. The DNA vector of claim 1 in which the T7 phage RNA polymerase promoter is fused downstream from the early immediate HCMV promoter region.

6. The DNA vector pCMX:

NGF-myc.

7. A DNA vector comprising pCMX:

NGF-myc.