Ligand binding assay for an analyte using surface-enhanced scattering (SERS) signal
First Claim
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1. A method for determining the presence or amount of an analyte in a test sample, comprising:
- (a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture;
said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent and an ancillary specific binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members;
said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing surface-enhanced Raman scattering (SERS), wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent;
(b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture;
(c) inducing a Raman scattering signal by illuminating said surface with a radiation;
(d) measuring the intensity of said SERS signal resulting from said illumination of said surface; and
(e) determining thereby the presence or amount of analyte in said test sample.
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Abstract
An assay for an analyte in a test sample that uses a ligand binding reaction between the analyte and a specific binding member or a binding member pair that includes one binding member having a Raman active reporter. The analyte can be assayed by measurement of the Raman scattering signal of the Raman-active reporter. The Raman scattering signal can be either a noon-enhanced Raman scattering signal or a Raman scattering signal that is enhanced by a surface capable of enhancing that Raman scattering signal.
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Citations
19 Claims
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1. A method for determining the presence or amount of an analyte in a test sample, comprising:
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(a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture;
said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent and an ancillary specific binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members;
said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing surface-enhanced Raman scattering (SERS), wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent;(b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) inducing a Raman scattering signal by illuminating said surface with a radiation; (d) measuring the intensity of said SERS signal resulting from said illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for performing a ligand-binding assay for determining the presence or amount of an analyte in a test sample, comprising:
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(a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture;
said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering;
wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent, and an ancillary binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members;
said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing a surface-enhanced Raman scattering (SERS) wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent;(b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) illuminating said surface with a radiation so as to induce a SERS signal; (d) measuring the intensity of Raman shift of said SERS signal resulting from the illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification