Microbioelectrode and method of fabricating the same

• US 5,269,903 A
• Filed: 10/22/1991
• Issued: 12/14/1993
• Est. Priority Date: 03/13/1987
• Status: Expired due to Fees

• Current Assignee: Shigeru Yamuchi, Yoshito Ikariyama
• Original Assignee: Shigeru Yamuchi, Yoshito Ikariyama
• Inventors: Yamuchi, Shigeru, Ikariyama, Yoshito
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US07/779,194
• Time in Patent Office: 784 Days
• Field of Search: 204/403, 204/415, 435/817, 435/288
• US Class Current: 204/403.11
• CPC Class Codes: C12N 11/14 Enzymes or microbial cells immobilised on or in an inorganic carrier
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Fine particles 1 of an electrically conductive material such as platinum black are being deposited electrolytically on the end surface of an electrically conductive material (base) such as platinum, with immersion of the conductive material in a solution containing both of a conductive material to produce porous deposition 1, e.g. platinum black, and a biofunctional material (biologically active substance) 2 such as an enzyme, so as to produce a microbioelectrode 7 having fine particle layer 1 and biologically active substance 2 incorporated therein, with significantly high surface area. This microbioelectrode can be compact in size and moreover, since the apparent surface area of the fine particles 1 of the conductive material is significantly large and a considerable amount of the biologically active material 2 can be incorporated and immobilized in the conductive fine particles 1, the intended trace target substance can be measured quickly and with high sensitivity even when it is in a trace amount of sample.

Claims

1. A biologically active substance immobilized microbioelectrode comprising a fine particle electrically conductive surface layer incorporating an immobilized biologically active substance formed by a single step of:

depositing fine particles of a conductive material and said active substance on the surface of an electroconductive material so as to form a porous conductive material incorporating said active substance therewithin.

2. The microbioelectrode in accordance with claim 1, further comprising the step of:

stabilizing the resulting active substance incorporated porous conductive material with a crosslinking agent.

3. The microbioelectrode in accordance with claim 2, further comprising the step of:

forming a polymeric film on the surface of said conductive materials for the stabilization of said active substance.

4. The microbioelectrode in accordance with claim 1, wherein said porous conductive material is treated by anodic oxidation after the deposition of said porous conductive material incorporating said active substance.

5. The microbioelectrode in accordance with claim 1, wherein said fine particles are deposited by a solution containing the conductive material.

6. The microbioelectrode in accordance with claim 1, wherein said fine particles are deposited by a chemical reaction utilized to generate the conductive material.

7. A microbioelectrode designed to have biologically active substances embodied directly in a transducing electrode, or immobilized in the fine pores or on the surface of a porous electroconductive material which is deposited on the surface of a transducing electrode, whereby both the biochemical reaction and electrochemical reaction occur simultaneously so as to enable direct transduction into an electric signal.

8. A microbioelectrode comprising a fine platinum black electrically conductive surface layer with an immobilized biologically active substance incorporated therein, wherein said microbioelectrode is formed by the single step of depositing particles of said fine platinum black and an active substance on the surface of an electroconductive material.

9. The microbioelectrode in accordance with claim 8, further comprising the step of:

stabilizing the resulting active substance incorporated porous conductive material with a crosslinking agent.

10. The microbioelectrode in accordance with claim 9, further comprising the step of:

forming a polymeric film on the surface of said conductive materials for the stabilization of said active substance.

11. The microbioelectrode in accordance with claim 8, wherein said porous conductive material is treated by anodic oxidation after the deposition of said porous conductive material incorporating said active substance.

12. The microbioelectrode in accordance with claim 8, wherein said fine particles are deposited by a solution containing the conductive material.

13. The microbioelectrode in accordance with claim 8, wherein said fine particles are deposited by a chemical reaction utilized to generate the conductive material.