Method for the selective cleavage of fusion proteins with lysostaphin
First Claim
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1. A method for the preparation of proinsulin by enzymatic cleavage of a fusion protein, wherein(a) a fusion protein is formed by linking proinsulin to a peptide as follows:
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space="preserve" listing-type="equation">(Y.sub.1 . . . Y.sub.m) - (Gly).sub.p+q - (X) where (Y1 . . . Ym) - (Gly)p represents the sequence which is to be eliminated and (Gly)q -(X) represents proinsulin;
X and Y denote, independently of one another, natural amino acids;
m is greater than 1;
p is greater than 0;
p+q is between 2 and 100, and if Ym is Gly, p+q can be less than 2, and p can be 0; and
(b) cleaving the fusion protein with lysostaphin.
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Abstract
The invention relates to a method for the preparation of polypeptides or proteins by enzymatic cleavage of the oligo- or polyglycine sequence of a fusion protein using an endoprotease.
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Citations
5 Claims
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1. A method for the preparation of proinsulin by enzymatic cleavage of a fusion protein, wherein
(a) a fusion protein is formed by linking proinsulin to a peptide as follows: -
space="preserve" listing-type="equation">(Y.sub.1 . . . Y.sub.m) - (Gly).sub.p+q - (X)where (Y1 . . . Ym) - (Gly)p represents the sequence which is to be eliminated and (Gly)q -(X) represents proinsulin; X and Y denote, independently of one another, natural amino acids; m is greater than 1; p is greater than 0; p+q is between 2 and 100, and if Ym is Gly, p+q can be less than 2, and p can be 0; and (b) cleaving the fusion protein with lysostaphin. - View Dependent Claims (2, 3, 4, 5)
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Specification