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Method for the selective cleavage of fusion proteins with lysostaphin

  • US 5,270,176 A
  • Filed: 06/29/1992
  • Issued: 12/14/1993
  • Est. Priority Date: 11/20/1987
  • Status: Expired due to Term
First Claim
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1. A method for the preparation of proinsulin by enzymatic cleavage of a fusion protein, wherein(a) a fusion protein is formed by linking proinsulin to a peptide as follows:

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    space="preserve" listing-type="equation">(Y.sub.1 . . . Y.sub.m) - (Gly).sub.p+q - (X) where (Y1 . . . Ym) - (Gly)p represents the sequence which is to be eliminated and (Gly)q -(X) represents proinsulin;

    X and Y denote, independently of one another, natural amino acids;

    m is greater than 1;

    p is greater than 0;

    p+q is between 2 and 100, and if Ym is Gly, p+q can be less than 2, and p can be 0; and

    (b) cleaving the fusion protein with lysostaphin.

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