Cell analysis apparatus

• US 5,270,212 A
• Filed: 02/26/1992
• Issued: 12/14/1993
• Est. Priority Date: 03/20/1991
• Status: Expired due to Fees

• Current Assignee: Hitachi Instruments Engineering Company Limited, Hitachi, Ltd.
• Original Assignee: Hitachi Instruments Engineering Company Limited, Hitachi, Ltd.
• Inventors: Yamazaki, Isao, Miyake, Ryo, Tatara, Nobuyuki, Ohki, Hiroshi, Kaneko, Toshio, Horiuchi, Hideyuki, Yabe, Ryohei, Sakuraba, Shinichi
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US07/841,487
• Time in Patent Office: 657 Days
• Field of Search: 435/291, 435/289, 435/290, 435/312, 435/316, 435/317.1, 422/63, 422/64, 422/82.02, 422/82.05, 422/82.08, 422/103, 436/43, 436/45, 436/63, 73/864.83, 73/864.84, 73/61.59, 73/64.56, 73/863.73, 356/39, 356/244
• US Class Current: 436/45
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 2015/011 with lysing, e.g. of erythrocytes
• CPC Class Codes: G01N 2015/014 Reticulocytes
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

This invention relates to an apparatus for analyzing cells in a biological liquid sample, such as blood, in which apparatus a flow passage in a sample pretreatment system can be shortened with a simple construction. A sample metering chamber, a mixing chamber and a measurement liquid extracting chamber are formed in a rotary sliding member of a pretreatment portion. The rotary sliding member is intermittently rotated so as to shift a sample from one chamber to another to perform a necessary treatment. Fluorescence or scattering light with respect to the sample, mixed with a reagent in the pretreatment portion, is detected by a flow cell.

Claims

1. A cell analysis apparatus for effecting multiple types of sample treatments to multiple samples, comprising:

a rotor radially divided equally into a plurality of segments of the same angle, each of said segments having a plurality of chambers therein of identical construction;

a pair of stators sandwiching said rotor;

means for providing each of a plurality of different samples to a chamber of a different one of the segments;

means for intermittently rotating the rotor through an angle corresponding to one-half of one segment to bring a sample in a segment to a treatment position corresponding to a position on one of said stators; and

means for performing one of a plurality of different sample treatments for each of the plurality of different samples, including means for performing a first sample treatment and thereafter performing a second sample treatment different from the first sample treatment following each angular rotation of the rotor.

2. A cell analysis apparatus according to claim 1, in which said plurality of different sample treatments includes at least one of the group consisting of sample suction, divided pouring, dilution, staining, agitation, measurement of sample parameters and washing.

3. A cell analysis apparatus according to claim 2, wherein the sample providing means includes sample suction means for receiving a sample by suction and for delivering a sample to one of the chambers in said segments, said sample suction means being directly provided on one of said stators.

4. A cell analysis apparatus according to claim 2, further comprising a sample measuring position, directly provided on one of said stators, at which a sample parameter of a sample drawn from one of said rotor segment chambers is measured.

5. A cell analysis apparatus according to claim 4, further comprising a plurality of sample measuring positions, directly provided on said one stator, at which different sample treatments are respectively performed on respective samples drawn from respective rotor segment chambers.

6. A cell analysis apparatus according to claim 5, wherein at least one of said sample measurement positions is located a distance in a rotational direction from another of said measuring positions, said distance corresponding to a reaction time of a treatment, when said reaction times are different for different sample treatments.

7. A cell analysis apparatus according to claim 2, wherein said chambers include a dilution chamber and a divided pouring chamber, and said apparatus further comprises a quantitative analysis flow cell operably associated with one of the stators for receiving a sample from one of the rotor segment chambers, said dilution chamber having a volume that is larger than the volume of the divided pouring chamber and the quantitative analysis flow cell.

8. A cell analysis apparatus according to claim 1, in which the locations of said plurality of chambers in the segments correspond to treating portions in which said plurality of sample treatments are to be effected, so that said samples are shifted from one of said chambers to another of said chambers within the same segment with each angular rotation of the rotor of a predetermined angle in a predetermined direction.

9. A cell analysis apparatus according to claim 1, further comprising a plurality of sample measuring positions, directly provided on one of said stators, at which different sample treatments are respectively performed on respective samples drawn from respective rotor segment chambers, wherein at least one of said sample measuring positions is located a distance in a rotational direction from another of said measuring positions, said distance corresponding to a reaction time of a treatment, when sample reaction times are different for different sample treatments.

10. A cell analysis apparatus according to claim 1, wherein at least one segment includes a first portion and a second portion, and further comprising means for providing a sample treatment to a sample contained in a chamber located in the first portion of said at least one segment at a treatment position and means for washing a chamber located in the second portion of said at least one segment at a washing position while said first position is at said treatment position.

11. A cell analysis apparatus according to claim 1, wherein at least one segment includes a first portion and a second portion, and further comprising means for simultaneously performing a different analysis for each of a sample contained in a chamber located in the first portion of said at least one segment at a first treatment position, and a sample contained in a chamber located in the second portion of said at least one segment at a second treatment position while said first portion is at said first treatment position.

12. A method of cell analysis for effecting multiple kinds of sample treatments to a sample by rotating a rotor sandwiched by two stators so as to change the sample treatments, comprising the steps of:

providing a rotor that is radially divided equally into a plurality of segments of the same angle, each of said segments having a plurality of chambers therein of identical construction;

providing a plurality of different samples, one sample to a respective chamber of one of said equally divided segments;

performing one of a plurality of different sample treatments for each of the plurality of different samples; and

intermittently rotating said rotor through an angle corresponding to one-half of one segment;

wherein said plurality of sample treatments are changed with each angular rotation of the rotor.

13. A method of cell analysis according to claim 12, wherein each of said plurality of different samples is a blood sample.

14. A method of cell analysis according to claim 12, in which said plurality of different sample treatments include at least one from the group consisting of blood corpuscle counting, leukocyte classifying, and reticulocyte counting.