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Apparatus for measuring polarization of bathochromically shifted fluorescence

  • US 5,270,788 A
  • Filed: 12/16/1991
  • Issued: 12/14/1993
  • Est. Priority Date: 05/27/1986
  • Status: Expired due to Fees
First Claim
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1. Apparatus for the measurement of the polarization value, P, of a fluorescing material in a sample comprising of fluorescing material and background material, the background material contributing background fluorescence, where the emission spectrum of the background fluorescence is shifted relative to the emission spectrum of the fluorescence from the fluorescing material, the apparatus comprising:

  • (a) an excitation source for exciting a sample comprising a fluorescing material and background material, the background contributing background fluorescence, at a selected excitation wavelength;

    (b) a fixed polarizer transmitting to the sample only plane-polarized light, the polarizer being disposed between the excitation source and the sample;

    (b) a fixed polarizer transmitting to the sample only plane-polarized light, the polarizer being disposed between the excitation source and the sample;

    (c) orientation selection means for selectively transmitting plane-polarized light in either a first plane parallel to the plane of polarization of the exciting light or a second plane transverse to the first plane, the orientation selection means disposed in the light path of the fluorescence emitted by the sample;

    (d) wavelength selection means for selecting either a primary wavelength, λ

    1, or a secondary wavelength, λ

    2, for subsequent fluorescence emission intensity measurements, the wavelength selection means being disposed in the path of the light emitted from the orientation selection means;

    (e) measuring means for measuring the intensities of the components of the emitted fluorescence polarized in the first and second planes of the at least two wavelengths selected by the wavelength selection means; and

    (f) calculation means for calculating the net polarization value, P, of the fluorescing material in the sample from the measure intensities at the at least two wavelengths.

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