Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
First Claim
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1. A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt'"'"'s lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
- (a) obtaining a biological sample having nucleated cells from a human;
(b) isolating said nucleated cells from said biological sample;
(c) isolating DNA from said nucleated cells;
(d) digesting said DNA with the restriction enzyme HindIII to obtain DNA fragments;
(e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments;
(f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly(ADP-ribose) polymerase wherein said probe will also hybridize to at least one DNA fragment of about 2.5 to about 2.7 kilobases in a human with a predisposition for cancer.
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Abstract
The invention relates to a method for detecting the presence of a DNA polymorphism associated with predisposition for certain cancers in a human. This method involves analyzing the human chromosome 13 using a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein the probe is capable of identifying the DNA polymorphism.
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Citations
20 Claims
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1. A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt'"'"'s lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
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(a) obtaining a biological sample having nucleated cells from a human; (b) isolating said nucleated cells from said biological sample; (c) isolating DNA from said nucleated cells; (d) digesting said DNA with the restriction enzyme HindIII to obtain DNA fragments; (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments; (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly(ADP-ribose) polymerase wherein said probe will also hybridize to at least one DNA fragment of about 2.5 to about 2.7 kilobases in a human with a predisposition for cancer. - View Dependent Claims (4, 5, 6)
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2. A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt'"'"'s lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
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(a) obtaining a biological sample having nucleated cells from a human; (b) isolating said nucleated cells from said biological sample; (c) isolating DNA from said nucleated cells; (d) digesting said DNA with the restriction enzyme PstI to obtain DNA fragments; (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments; (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly(ADP-ribose) polymerase wherein said probe will also hybridize to a DNA fragment of about 5.7 kilobases to about 6.1 kilobases in a human with a predisposition for cancer.
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3. A method for detecting a DNA polymorphism associated with predisposition for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt'"'"'s lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
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(a) obtaining a biological sample having nucleated cells from a human; (b) isolating said nucleated cells from said biological sample; (c) isolating DNA from said nucleated cells; (d) digesting said DNA with the restriction enzyme EcoR1 to obtain DNA fragments; (e) separating fragments obtained from said digestion according to their respective molecular weights to form a pattern for said fragments; (f) detecting a DNA polymorphism associated with predisposition for cancer in a human with a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein said probe will also hybridize to a DNA fragment of about 5.1 kilobases in a human with a predisposition for cancer.
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7. A method for detecting susceptibility for cancer in a human, wherein said cancer is selected from the group consisting of Burkitt'"'"'s lymphoma, B follicular cell lymphoma, acute myeloid leukemia, chronic myeloid leukemia, small cell lung carcinoma, and breast adenocarcinoma, comprising the steps of:
identifying the presence of a DNA polymorphism on chromosome 13 associated with susceptibility for cancer using a hybridisation probe which will hybridize to the gene for human poly (ADP-ribose) polymerase or closely linked sequences on chromosome 13 wherein the probe is capable of identifying said DNA polymorphism. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 20)
- 16. A restriction fragment length polymorphic probe comprising nucleic acid sequences which will hybridize to the gene for poly (ADP-ribose) polymerase and will also detect a DNA polymorphism associated with a susceptibility for cancer on human chromosome 13.
Specification