Assay for serum cholinesterase activity
First Claim
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1. A method for assaying serum cholinesterase activity in an aqueous liquid comprising the steps of:
- A) contacting a sample of the aqueous liquid with an analytical element for assaying serum cholinesterase activity comprising a support having thereon at least one layer, wherein the element also comprises;
a) butyrylthiocholine;
b) ferricyanide at a coverage of about 0.02 to 2.0 g/m2;
c) a binder material at a coverage of about 3 to 12 g/m2;
andd) a buffer to establish a pH in the range of about 7.0-8.5; and
B) monitoring the rate of change in reflectance density to assay the activity of serum cholinesterase in the sample.
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Abstract
A dry analytical element for the determination of serum cholinesterase (CHE) activity is disclosed. The element analyses undiluted body fluids and employs butyrylthiocholine as the substrate for CHE. Butyrylthiocholine is hydrolyzed by serum cholinesterase and liberates butyric acid and thiocholine. The thiocholine liberated then reduces ferricyanide to ferrocyanide and the rate of change is measured by reflectance densitometry.
16 Citations
15 Claims
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1. A method for assaying serum cholinesterase activity in an aqueous liquid comprising the steps of:
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A) contacting a sample of the aqueous liquid with an analytical element for assaying serum cholinesterase activity comprising a support having thereon at least one layer, wherein the element also comprises; a) butyrylthiocholine; b) ferricyanide at a coverage of about 0.02 to 2.0 g/m2; c) a binder material at a coverage of about 3 to 12 g/m2;
andd) a buffer to establish a pH in the range of about 7.0-8.5; and B) monitoring the rate of change in reflectance density to assay the activity of serum cholinesterase in the sample. - View Dependent Claims (2, 3, 4, 5)
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6. An analytical element for assaying serum cholinesterase activity comprising a support having thereon at least one layer, wherein the element also comprises:
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a) butyrylthiocholine; b) ferricyanide at a coverage of about 0.02 to 2.0 g/m2 ; c) a binder material at a coverage of about 3 to 12 g/m2;
andd) a buffer to establish a pH in the range of about 7.0-8.5. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification