×

Method of preferential labelling of a phycobiliprotein with an amine-reactive dye for use in a multiple color assay and product for such use

  • US 5,272,257 A
  • Filed: 09/03/1992
  • Issued: 12/21/1993
  • Est. Priority Date: 05/21/1990
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for producing a phycobiliprotein/amine-reactive dye conjugate wherein said phycobiliprotein is labelled with said dye, said method comprising reacting an amine-reactive dye with a phycobiliprotein in the presence of 1 to 20% of salt which effects an intramolecular rearrangement of said phycobiliprotein to expose reactive amino groups in hydrophobic sites on said phycobiliprotein which reacts with said dye, removing excess dye and separating the conjugate by a chromatographic means.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×