Apparatus and method for in vitro manipulation of blood

• US 5,275,953 A
• Filed: 04/22/1991
• Issued: 01/04/1994
• Est. Priority Date: 04/22/1991
• Status: Expired due to Fees

• Current Assignee: Bull, Brian S.
• Original Assignee: Bull, Brian S.
• Inventors: Bull, Brian S.
• Primary Examiner(s): Johnston, Jill A.
• Application Number:
  US07/688,892
• Time in Patent Office: 988 Days
• Field of Search: 436/69, 436/70, 422/73, 422/100, 422/101, 128/763, 128/770, 128/653.4, 128/655, 424/4, 435/13
• US Class Current: 436/69
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/56
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors

Abstract

A blood extracting and receiving container assembly (10) having a coating agent (22) disposed on an interior surface of a blood receiving chamber (12) and an extracting apparatus (14) to effectively isolate a blood specimen from contacting surfaces which would initiate clotting. The coating agent contains molecules which inhibit the activation of blood elements responsible for initiating blood clotting processes. A method for manipulating a blood specimen in vitro in association with the subject blood extracting and receiving assembly (10) includes the steps of coating an interior surface of the extracting and receiving container assembly (10) with nonthrombogenic coating agent (22), and, while coating agent (22) adheres to the interior surface of assembly (10), withdrawing a blood specimen (24) into the assembly. Additionally, the method includes the steps of exposing the specimen to an activating surface in a controlled manner to initiate blood clotting processes, preferably while a portion of the specimen is admixed with the nonthrombogenic agent. A clotting parameter, such as weight or clotting rate, may be correlated with a disease condition.

Claims

1. A method for containing a blood specimen in vitro comprising the steps of:

providing an interior surface of a blood specimen extracting and receiving container assembly with a layer of an ionic radiographic contrast agent; and

while said agent is provided on said interior surface of said specimen extracting and receiving container assembly, withdrawing a blood specimen from a patient and depositing said specimen into said specimen extracting and receiving container assembly.

2. The method as defined in claim 1 wherein:

said providing step is accomplished by coating an interior surface of said blood specimen extracting and receiving container assembly with said agent.

3. The method as defined in claim 2 wherein:

said agent has a greater density and viscosity than blood.

4. The method as defined in claim 3 wherein:

after said withdrawing step, exposing said blood specimen to an activating means to initiate blood clotting, and

during said exposing step, mixing said blood specimen with said coating agent.

5. The method as defined in claim 4 wherein:

after said exposing step and said mixing step, quantitatively measuring clotted blood in said specimen.

6. The method as defined in claim 2, and the additional step of:

after said withdrawing step, exposing the blood specimen to an activating means in a controlled manner to initiate blood clotting.

7. The method as defined in claim 6, and the additional step of:

after said exposing step, correlating a clotting parameter with a disease condition.

8. The method as defined in claim 6 wherein:

said exposing step is accomplished by introducing a blood clot inducing means into said chamber of said blood extracting and receiving assembly.

9. The method as defined in claim 6 wherein:

after said exposing step, measuring the amount of clotting during the clotting process.

10. The method as defined in claim 9 wherein:

said measuring step is accomplished while the clotted blood remains within said chamber.

11. The method as defined in claim 6 wherein:

said exposing step is accomplished by periodically displacing a chamber of said specimen extracting and receiving container assembly until motion of the blood specimen in said chamber removes a portion of said coating agent from said interior surface of said chamber and said blood specimen contacts an uncoated area of said interior surface.

12. The method as defined in claim 11 wherein:

said specimen receiving chamber is an elongated tube; and

said displacing step is accomplished by mounting said elongated tube in a horizontal orientation and rotating said elongated tube about a longitudinal axis of said elongated tube.

13. The method as defined in claim 12 wherein:

said elongated tube is rotated by rolling said elongated tube along a horizontal plane by a distance so that any point on said elongated tube travels through an arc length subtended by an angle up to about 45°

.

14. The method as defined in claim 13 wherein:

said elongated tube is rolled in one direction and then rolled in an opposite direction over a roll cycle of approximately 25 seconds.

15. The method as defined in claim 12 wherein:

said elongated tube is rotated by tilting said tube about a tilt axis substantially parallel to said longitudinal axis.

16. The method as defined in claim 15 wherein,

said tilting step is accomplished by oscillatory tube supporting means carrying said tube about mid tilt axis by about 45°

above and about 45°

below a horizontal plane.

17. An assembly for maintaining blood in vitro in essentially the same rate as occurs in vivo comprising:

a chamber for receiving a blood specimen;

an ionic radiographic contrast agent provided as a coating on an interior surface of said chamber to prevent contact between the blood specimen and thrombotic initiating surface; and

a blood specimen contained in a volume of said chamber interiorly of said coating of said ionic radiographic contrast agent.

18. The assembly as defined in claim 17 wherein:

said agent is a liquid having a density and viscosity greater than blood.

19. The assembly as defined in claim 17 wherein:

said agent is a liquid having a viscosity greater than blood and a density less than blood.

20. The assembly as defined in claim 17, and

extracting means communicating with said chamber for the passage of blood into said chamber, said ionic radiographic contrast agent being provided as a coating on an interior surface of said extracting means.

21. A method for containing a blood specimen in vitro comprising the steps of:

coating an interior surface of at least a portion of a container assembly with an ionic radiographic contrast agent to prevent contact of said interior surface in said portion of said container assembly by said blood specimen; and

while said agent is coated on said interior surface, withdrawing a blood specimen from a patient into said portion of said container assembly.