Gel-in-matrix containing a fractured hydrogel

• US 5,277,915 A
• Filed: 03/27/1992
• Issued: 01/11/1994
• Est. Priority Date: 10/30/1987
• Status: Expired due to Term

• Current Assignee: Cambrex Bio Science Rockland, Inc. (Lonza Group AG)
• Original Assignee: FMC Corporation
• Inventors: Kirkpatrick, Francis H., Provonchee, Richard B.
• Primary Examiner(s): Kulkosky, Peter F.
• Application Number:
  US07/861,155
• Time in Patent Office: 655 Days
• Field of Search: 521/28, 521/55, 424/443, 424/439, 424/441, 424/444, 424/409, 424/485, 428/308.4, 428/289, 428/402, 428/403, 428/404, 428/407.3, 523/122
• US Class Current: 424/485
• CPC Class Codes: B01D 15/08 Selective adsorption, e.g. chromatography
• CPC Class Codes: B01D 15/34 Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
• CPC Class Codes: B01J 20/28026 Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin
• CPC Class Codes: B01J 20/28028 Particles immobilised within fibres or filaments
• CPC Class Codes: B01J 20/28033 Membrane, sheet, cloth, pad, lamellar or mat
• CPC Class Codes: B01J 20/28042 Shaped bodies; Monolithic structures
• CPC Class Codes: B01J 20/28045 Honeycomb or cellular structures; Solid foams or sponges
• CPC Class Codes: B01J 20/28047 Gels
• CPC Class Codes: B01J 20/28069 Pore volume, e.g. total pore volume, mesopore volume, micropore volume
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: B01J 47/018 Granulation; Incorporation of ion-exchangers in a matrix; Mixing with inert materials
• CPC Class Codes: C08J 2205/02 the finished foam itself being a gel or a gel being temporarily formed when processing the foamable composition
• CPC Class Codes: C08J 2403/00 Characterised by the use of starch, amylose or amylopectin or of their derivatives or degradation products
• CPC Class Codes: C08J 9/42 with macromolecular compounds
• CPC Class Codes: Y10T 428/249955 Void-containing component partially impregnated with adjacent component
• CPC Class Codes: Y10T 428/249958 Void-containing component is synthetic resin or natural rubbers
• CPC Class Codes: Y10T 428/249994 Composite having a component wherein a constituent is liquid or is contained within preformed walls [e.g., impregnant-filled, previously void containing component, etc.]
• CPC Class Codes: Y10T 428/2967 Synthetic resin or polymer
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]
• CPC Class Codes: Y10T 442/2033 Coating or impregnation formed in situ [e.g., by interfacial condensation, coagulation, precipitation, etc.]

Abstract

A three dimensional porous matrix, such as a reticulated open-cell foam, having within its matrix structure a mechanically-fractured hydrogel containing a network of fracture channels. The mechanically-fractured hydrogel may be a gel that has been partially dewatered by compression of the gel in situ.

The porosity of the gel-in-matrix makes it useful for chromatographic applications and for immobilization of biologically-active components, where efficient, intimate contact of the hydrogel with a liquid medium is important.

Claims

1. A gel-in-matrix combination comprising a three-dimensional porous support matrix and a porous fractured hydrogel fixedly disposed therein, in which said fractured hydrogel has:

(a) a void volume of about 10-90%; and

(b) a plurality of porous fractures comprising channels having a mean characteristic dimension of about 0.1-1,000 μ

m.

2. The gel-in-matrix of claim 1 wherein said fractured hydrogel void volume is about 50-90%.

3. The gel-in-matrix of claim 1 wherein said hydrogel fracture channels have a mean characteristic dimension of about 1-100 μ

m.

4. The gel-in-matrix of claim 2 wherein said hydrogel fracture channels have a mean characteristic dimension of about 1-100 μ

m.

5. The gel-in-matrix of claim 1 wherein said hydrogel is thermally reversible to a liquid.

6. The gel-in-matrix of claim 1 wherein said hydrogel is a polysaccharide which is thermally reversable to a liquid.

7. The gel-in-matrix of claim 1 wherein said hydrogel is an alginate, carrageenan, agar, agarose, curdlan, pullulan, gellan, a derivatized composition of any of the foregoing, or a mixture thereof.

8. The gel-in-matrix of claim 3 wherein said hydrogel is an alginate, carrageenan, agar, agarose, a derivatized composition of any of the foregoing, or a mixture thereof.

9. The gel-in-matrix of claim 4 wherein said hydrogel is an alginate, carrageenan, agar, agarose, a derivatized composition of any of the foregoing, or a mixture thereof.

10. The gel-in-matrix of claim 1 wherein said hydrogel is an agarose or derivatized agarose.

11. The gel-in-matrix of claim 4 wherein said hydrogel is an agarose or a derivatized agarose.

12. The gel-in-matrix of claim 1 wherein said hdyrogel comprises about 0.05-10% w/w gel-forming component based upon the total gel.

13. The gel-in-matrix of claim 11 wherein said hdyrogel comprises about 0.05-10% w/w gel-forming component based upon the total gel.

14. The gel-in-matrix of claim 1 wherein said matrix comprises a material inert with respect to the hydrogel, having pores with a mean characteristic dimension of less than about 2 mm.

15. The gel-in-matrix of claim 13 wherein the matrix comprises a material inert with respect to the hydrogel, having pores with a mean characteristic dimension of less than about 2 mm.

16. The gel-in-matrix of claim 1 wherein said matrix is hydrophilic.

17. The gel-in-matrix of claim 15 wherein said matrix is hydrophilic.

18. The gel-in-matrix of claim 1 wherein aid matrix is a porous:

plastic, ceramic, metal, flexible plastic foam, rigid plastic foam, or mass of nonwoven fibers.

19. The gel-in-matrix of claim 17 wherein said matrix is a porous:

plastic, ceramic, metal, flexible plastic foam, rigid plastic foam, or mass of nonwoven fibers.

20. The gel-in-matrix of claim 1 wherein said matrix is a reticulated open-cell porous plastic foam.

21. The gel-in-matrix of claim 19 wherein said matrix is a reticulated open-cell porous plastic foam.

22. The gel-in-matrix of claim 1 wherein said matrix is a polyurethane foam.

23. The gel-in-matrix of claim 21 wherein said matrix is a polyurethane foam.

24. The gel-in-matrix of claim 1 wherein said matrix contains about 20-200 pores per square inch.

25. The gel-in-matrix of claim 23 wherein said matrix contains about 20-200 pores per square inch.

26. The gel-in-matrix of claim 1 wherein said matrix is a mass of nonwoven fibers.

27. The gel-in-matrix of claim 19 wherein said matrix is a mass of nonwoven fibers.

28. The gel-in-matrix of claim 1 wherein said composition is in the form of a thin sheet, a strand, a cylindrical plug, a cylindrical annulus, a disc, or beads.

29. The gel-in-matrix of claim 19 wherein said composition is in the form of a thin sheet, a strand, a cylindrical plug, a cylindrical annulus, a disc, or beads.

30. The gel-in-matrix of claim 25 wherein said composition is in the form of a thin sheet, a strand, a cylindrical plug, a cylindrical annulus, a disc, or beads.

31. The gel-in-matrix of claim 27 wherein said composition is in the form of a thin sheet, a strand, a cylindrical plug, a cylindrical annulus, a disc, or beads.

32. The gel-in-matrix of claim 1 wherein said hydrogel further comprises at least one buffer, conventional hydrogel additive, or organic solvent in an amount or of a type which is miscible with an aqueous medium from which said hydrogel has been formed.

33. The gel-in-matrix of claim 1 wherein said hydrogen further comprises a solid or immiscible liquid immobilized within it.

34. The gel-in-matrix of claim 19 wherein said hydrogel further comprises a solid or immiscible liquid immobilized within it.

35. The gel-in-matrix of claim 21 wherein said hydrogel further comprises a solid or immiscible liquid immobilized within it.

36. The gel-in-matrix of claim 23 wherein said hdyrogel further comprises a solid or immiscible liquid immobilized within it.

37. The gel-in-matrix of claim 27 wherein said hydrogel further comprises a solid or immiscible liquid immobilized within it.

38. The gel-in-matrix of claim 1 wherein said hydrogel contains at least one biologically active component immobilized within it.

39. The gel-in-matrix of claim 4 wherein said hydrogel contains at least one biologically active component immobilized within it.

40. The gel-in-matrix of claim 9 wherein said hydrogel contains at least one biologically active component immobilized within it.

41. The gel-in-matrix of claim 13 wherein said hydrogel contains at least one biologically active component immobilized within it.

42. The gel-in-matrix of claim 19 wherein said hydrogel contains at least one biologically active component immobilized within it.

43. The gel-in-matrix of claim 25 wherein said hydrogel contains at least one biologically active component immobilized within it.

44. The gel-in-matrix of claim 30 wherein said hydrogel contains at least one biologically active component immobilized within it.

45. A method for manufacturing the gel-in-matrix of claim 1 comprising creating the fractures in said gel by partially dewatering said hydrogel in situ within said matrix.

46. The method of claim 45 wherein said hydrogen is dewatered until it contains about 10-90% of its original weight prior to said dewatering.

47. The method of claim 45 wherein said hydrogel is dewatered until it contains about 15-50% of its original weight prior to said dewatering

48. A gel-in-matrix produced by the method of claim 45.

49. The gel-in-matrix produced by the method of claim 46.

50. A gel-in-matrix produced by the method of claim 47.