Soybean peroxidase assays
First Claim
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1. An analytical composition comprising soybean peroxidase or a soybean peroxidase-antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide.
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Abstract
An analytical composition comprising antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide assays performed using this composition are described.
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13 Claims
- 1. An analytical composition comprising soybean peroxidase or a soybean peroxidase-antibody conjugate, an indicator which is capable of providing a detectable condition in the presence of peroxidase and hydrogen peroxide.
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5. An analytical element comprising an adsorbent carrier material containing soybean peroxidase or a soybean peroxidase antibody conjugate.
- 6. A method for the determination of an analyte comprising the steps of contacting a sample of a liquid suspected of containing an analyte with soybean peroxidase or a soybean peroxidase-labeled antibody, an indicator which is capable of providing a detectable change in the presence of peroxidase and hydrogen peroxide, and determining said detectable change as a result of the presence of said analyte.
- 8. A method for detecting an antigen which includes the steps of adding soybean peroxidase to a sample suspected of containing the antigen to be detected under conditions such that said peroxidase directly or indirectly bonds to said antigen via an antibody and adding a substrate for said peroxidase and an indicator to said sample under conditions such that said peroxidase and said substrate react with said indicator and produce a change which is an indication of the presence and/or amount of said antigen.
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12. A soybean peroxidase-antibody conjugate.
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13. A soybean peroxidase-antisoybean peroxidase antibody complex.
Specification