Automated miniature centrifuge

• US 5,279,150 A
• Filed: 03/13/1992
• Issued: 01/18/1994
• Est. Priority Date: 03/13/1992
• Status: Expired due to Fees

• Current Assignee: Internal Revenue Service (Government of the United States of America)
• Original Assignee: Albert E. Katzer, Charles F. Mcbrairty, Rodney A. Katzer
• Inventors: Katzer, Albert E., McBrairty, Charles F., Katzer, Rodney A.
• Primary Examiner(s): Williams, Hezron E.
• Assistant Examiner(s): BROCK, MICHAEL J
• Application Number:
  US07/851,183
• Time in Patent Office: 676 Days
• Field of Search: 73/61.63, 73/61.65, 73/61.66, 73/61.68, 73/61.69, 73/61.71, 73/64.56, 73/61.41, 73/61.43, 250/265, 250/273, 250/275, 250/276, 250/277, 356/39, 356/435, 356/436, 356/246, 356/440, 356/428, 436/10, 436/16, 436/45, 436/70, 436/72, 422/101, 422/82.05, 422/82.09, 128/760, 128/770, 604/317, 494/10, 494/16
• US Class Current: 73/61.66
• CPC Class Codes: G01N 15/042 by centrifuging and investigating centrifugates centrifuges per se B04B
• CPC Class Codes: G01N 2015/045 by optical analysis
• CPC Class Codes: G01N 2015/055 for hematocrite determination
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 9/30 by using centrifugal effects
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

A measurement device is provided for determining relative proportions of at least two materials forming a liquid and having different densities and different responses to light. A transparent capillary size cuvette tube confines the sample liquid, and is attached radially on a rotor wheel, preferably by a holder which seals an outer end of the tube and provides a package for manipulation of the tube without exposure to the sample. A motor controlled by a processor turns the rotor wheel, separating the materials by their densities. A linear light source illuminates the tube, and a linear array of photosensors defining pixels produces a signal which varies where the different materials abut. The pixels are counted, to locate the stratum between materials and the result is displayed as a percentage of total volume, suitable for hematocrit measurements. The cuvette tube has a fin for assisting in manipulation, is shaped as an elongated lens, and is cut to a spherical end surface to assist in sample collection.

Claims

1. A measurement apparatus for determining relative proportions of at least two materials forming a liquid, the at least two materials having different densities and different light transmission properties, the apparatus comprising:

at least once container for confining a sample of the liquid, the container being light transmissive and having an upper surface, a lower surface and an internal bore, one of said upper and lower surfaces being convex and the other being substantially flat;

a rotor wheel having means for holding the container such that the container occupies a length extension radially of an axis of rotation of the rotor wheel, and is held radially inwardly relative to the axis, at an elongated radial opening in the rotor wheel;

a motor coupled to the rotor wheel for rotating the rotor wheel and the container about the axis, whereby the materials of different densities are separated along the length extension due to centripetal acceleration relative to the axis, substantially to occupy different positions along the length extension, thereby forming an interface between the materials;

at least one source of radiation arranged to illuminate said container, incident on the convex one of said upper and lower surfaces of the container, along at least a portion of the length extension;

radiation sensing means mounted adjacent the flat one of said upper and lower surfaces of the container, operable to detect radiation from the source over a range encompassing at least a part of said portion of the length extension, the radiation sensing means comprising an elongated array of pixel elements producing a distinct signal with variation of detected radiation on opposite sides of the interface due to said different light transmission properties of the materials; and

,

indicator means responsive to the signal for displaying a relative position of the interface in the range.

2. The measurement apparatus according to claim 1, wherein the pixel elements are photodiodes defining an array, and further comprising at least one amplifier coupled to the photodiodes and to at least one threshold detector operable to compare a signal level for each of the photodiodes to a threshold level for discriminating high and low light levels.

3. The measurement apparatus according to claim 2, further comprising a digital processor and timing means responsive to the signal, operable to count pixels on at least one side of a change between high and low light levels occurring at the interface, and to calculate the percentage of the range occupied by pixels on that side relative to a total number in the range.

4. The measurement apparatus according to claim 3, wherein the indicator means comprises a numeric display operable to present the percentage.

5. The measurement apparatus according to claim 1, further comprising means operable to sense an angular position of the rotor, for triggering the radiation sensing means at a position where the opening is disposed between the source of radiation and the radiation sensing means.

6. The measurement apparatus of claim 1, wherein the container is a capillary tube cuvette that is elongated along the length extension, and has an inner diameter that is sufficiently small to retain the liquid via surface tension of the liquid, and wherein the means for holding the container includes a cuvette holder operable to seal at least a radially outer end of the cuvette, the rotor having means for engaging the cuvette holder to position the cuvette along the radial extension.

7. The measurement apparatus according to claim 6, wherein at least one end of the cuvette is shaped to define part of a spherical surface adjacent to a capillary inlet to the cuvette.

8. The measurement apparatus according to claim 1, wherein the flat surface of the container is oriented toward the radiation sensing means and the convex surface of the container is oriented toward the source of illumination, and further comprising a sensor operable to trigger the radiation sensing means at an angular position of the rotor where the container is between the source of illumination and the radiation sensing means.

9. A measurement apparatus for determining relative proportions of at least two materials forming a liquid, the at least two materials having different densities and different properties of at least one of light transmission and light reflection, the apparatus comprising:

at least one container for confining a sample of the liquid, the container comprising a light transmissive cuvette having a fin enabling manual manipulation of the cuvette;

a rotor wheel having means for holding the container such that the container occupies a length extension radially of an axis of rotation of the rotor wheel, and is held radially inwardly relative to the axis;

a motor coupled to the rotor wheel for rotating the rotor wheel and the container about the axis, whereby the materials of different densities are separated along the length extension due to centripetal acceleration relative to the axis, substantially to occupy different positions along the length extension, thereby forming an interface between the materials;

at least one source of radiation arranged to illuminate the materials in said container along at least a portion of the length extension;

radiation sensing means operable to detect radiation from the source over a range encompassing at least a part of said portion of the length extension, the radiation sensing means producing a distinct signal with variation of detected radiation on opposite sides of the interface due to said different properties of the materials; and

,

indicator means responsive to the signal to displaying a relative position of the interface in the range.

10. The measurement apparatus according to claim 9, wherein the fin is arranged at one end of the cuvette and tapers toward the cuvette approaching an opposite end of the cuvette.