Emission based fiber optic sensors for pH and carbon dioxide analysis

• US 5,280,548 A
• Filed: 03/11/1993
• Issued: 01/18/1994
• Est. Priority Date: 03/11/1993
• Status: Expired due to Fees

• Current Assignee: Ohmeda Pte Limited
• Original Assignee: BOC Health Care Incorporated (General Electric Company)
• Inventors: Atwater, Beauford W., Laksin, Olga
• Primary Examiner(s): Gonzalez, Frank
• Assistant Examiner(s): BARNS, STEPHEN
• Application Number:
  US08/003,090
• Time in Patent Office: 313 Days
• Field of Search: 385/12, 385/143
• US Class Current: 385/12
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/221 for investigating pH value

Abstract

The present invention is directed to a fiber optic sensor for analyzing pH which comprises (a) a fluorescence-based fiber optic sensor; and (b) a fluorescent dye-polymeric matrix in contact with the sensor, wherein the fluorescent dye is seminaphthorhodaflor-1-isothiocyanate, the polymer is a hydrogel selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, dextrans, and polysaccharides, and the dye is chemically bonded to the polymer. The present invention is also directed to a fiber optic sensor for analyzing carbon dioxide which comprises (a) a fluorescence-based fiber optic sensor; and (b) a fluorescent dye-polymeric-silicone elastomeric matrix in contact with the sensor, wherein the matrix comprises a fluorescent dye-polymeric premixture in a silicone elastomer, and the fluorescent dye is selected from the group consisting of seminaphthorhodaflor-1-carboxylate (SNARF-1C), seminaphthorhodaflor-1-isothiocyanate (SNARF-1C-ITC), seminaphthorhodaflor-1-dextran conjugate (SNARF-1C-Dex), 10-diethylamino seminaphthorhodaflor-1-carboxylate (SNARF-2C), and seminaphthorhodaflor-1-carboxylate 10-acetate (SNAFL-1C diacetate), the polymer is a hydrogel selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, dextrans, and polysaccharides, and the silicone elastomer is a polydimethyl siloxane selected from the group consisting of hydroxy, epoxy, and vinyl terminated polydimethyl siloxanes.

Claims

1. A fiber optic sensor for analyzing pH which comprises:

(a) a fluorescence-based fiber optic sensor; and

(b) a fluorescent dye-polymeric matrix in contact with the sensor, wherein the fluorescent dye is seminaphthorhodaflor-1-isothiocyanate, the polymer is a hydrogel selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, dextrans, and polysaccharides, and the dye is chemically bonded to the polymer.

2. The fiber optic sensor according to claim 1, wherein the polymer is selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, and dextrans.

3. The fiber optic sensor according to claim 2, wherein the polymer is selected from the group consisting of polyhydroxyethylmethacrylate and polyvinylpyrrolidone.

4. The fiber optic sensor according to claim 1, wherein the fluorescent dye is present in the fluorescent dye-polymeric matrix in an amount from about 0.01% to about 1%, by weight.

5. The fiber optic sensor according to claim 4, wherein the fluorescent dye is present in the fluorescent dye-polymeric matrix in an amount from about 0.01% to about 0.5%, by weight.

6. The fiber optic sensor according to claim 1, wherein the fluorescent dye-polymeric matrix has a pK_a in the range from about 6.5 to about 10.

7. A fiber optic sensor for analyzing carbon dioxide which comprises:

(a) a fluorescence-based fiber optic sensor; and

(b) a fluorescent dye-polymeric-silicone elastomeric matrix in contact with the sensor, wherein the matrix comprises a fluorescent dye-polymeric premixture in a silicone elastomer, and the fluorescent dye is selected from the group consisting of seminaphthorhodaflor-1-carboxylate (SNARF-1C)), seminaphthorhodaflor-1-isothiocyanate (SNARF-1C-ITC), seminaphthorhodaflor-1-dextran conjugate (SNARF-1C-Dex), 10-diethylamino seminaphthorhodaflor-1-carboxylate (SNARF-2C), and seminaphthorhodaflor-1-carboxylate 10-acetate (SNAFL-1C diacetate), the polymer is a hydrogel selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, dextrans, and polysaccharides, and the silicone elastomer is a polydimethyl siloxane selected from the group consisting of hydroxy, epoxy, and vinyl terminated polydimethyl siloxanes.

8. The fiber optic sensor according to claim 7, wherein the fluorescent dye is selected from the group consisting of seminaphthorhodaflor-1-carboxylate, seminaphthorhodaflor-1-isothiocyanate, and seminaphthorhodaflor-1-dextran conjugate.

9. The fiber optic sensor according to claim 8, wherein the fluorescent dye is seminaphthorhodaflor-1-carboxylate.

10. The fiber optic sensor according to claim 7, wherein the polymer is selected from the group consisting of polyhydroxyethylmethacrylate, polyvinylpyrrolidone, cellulose, and dextrans.

11. The fiber optic sensor according to claim 10, wherein the polymer is selected from the group consisting of polyhydroxyethylmethacrylate and polyvinylpyrrolidone.

12. The fiber optic sensor according to claim 7, wherein the silicone elastomer is selected from the group consisting of epoxy and vinyl terminated polydimethyl siloxanes.

13. The fiber optic sensor according to claim 12, wherein the silicone elastomer is a vinyl terminated polydimethyl siloxane.

14. The fiber optic sensor according to claim 7, wherein the fluorescent dye is present in the fluorescent dye-polymeric premix in an amount from about 0.1% to about 10%, by weight.

15. The fiber optic sensor according to claim 14, wherein the fluorescent dye is present in the fluorescent dye-polymeric premix in an amount from about 0.5% to about 5%, by weight.

16. The fiber optic sensor according to claim 7, wherein the fluorescent dye-polymeric premix is present in the fluorescent dye-polymeric-silicone elastomeric matrix in an amount from about 1% to about 30%, by weight.

17. The fiber optic sensor according to claim 16, wherein the fluorescent dye-polymeric premix is present in the fluorescent dye-polymeric-silicone elastomeric matrix in an amount from about 5% to about 25%, by weight.

18. The fiber optic sensor according to claim 7, wherein the fluorescent dye-polymeric premixture has a pK_a in the range from about 7 to about 9.

19. The fiber optic sensor according to claim 7, wherein the fluorescent dye-polymeric premixture is present in the silicone elastomer as a fine dispersion in the range of about 1 to 10 microns.