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Test array for performing assays

  • US 5,281,540 A
  • Filed: 11/22/1991
  • Issued: 01/25/1994
  • Est. Priority Date: 08/02/1988
  • Status: Expired due to Fees
First Claim
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1. An apparatus for supporting a plurality of selected capture reagents, each reactive with a specific assay binding component of interest in a biological sample, for use in simultaneously assaying the sample for said assay binding components, comprising:

  • a first layer of capture reagent binding material;

    a second layer of non-absorbent substrate material below the first layer;

    a plurality of test sites formed in said first layer of capture reagent binding material each adapted to bind a selected capture reagent; and

    isolation means for isolating each test site from every other test site and for confining capture reagent applied to one of said test sites, wherein said isolation means includes a plurality of isolation moats formed by depressions in said first layer and disposed about said test sites in said first layer, and wherein said depressions of said first layer material are hydrophobic.

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