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Compositions for and detection of human papillomavirus by specific oligonucleotide polymerase primers using the polymerase chain reaction

  • US 5,283,171 A
  • Filed: 02/15/1991
  • Issued: 02/01/1994
  • Est. Priority Date: 09/09/1988
  • Status: Expired due to Fees
First Claim
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1. A method for detecting genital human papillomavirus (HPV) in a sample and typing the HPV, if present, comprising:

  • (a) treating the sample with a pair of consensus HPV primers, DNA polymerase, and deoxynucleoside 5'"'"'-triphosphates under conditions such that an extension product of a consensus primer can be synthesized if HPV is present, wherein said consensus primers are a mixture of oligonucleotides that comprises at least a pair of primers sufficiently complementary to separate strands of HPV DNA to hybridize thereto so that the extension product synthesized from one member of said pair, when separated from its complementary strand, can serve as a template for synthesis of the extension product of the other member of said pair, said pairs of consensus primers selected from the group consisting of (a) FS10, JS15 and JS16;

    (b) FS17, JS15 and JS16; and

    (c) MY01, JS15 and JS16;

    (b) separating the primer extension products, if present, from the templates on which the extension products were synthesized to form single-stranded molecules;

    (c) treating the single-stranded molecules generated in step (b), if any, with the consensus primers of step (a) under conditions such that a primer extension product is synthesized using each of the single-stranded molecules produced in step (b) as a template;

    (d) repeating steps (b) and (c) at least once;

    (e) determining of amplification has occurred; and

    , if amplification has occurred,(f) hybridizing a type-specific DNA probe to said amplified DNA; and

    (g) determining if hybridization has occurred.

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