Preparation of a compressed membrane containing immobilized biologically acting material
First Claim
1. A process for making a porous carrier containing an immobilized protein, which comprises:
- attaching a plurality of polar functional groups to an internal surface which defines a plurality of micropores distributed throughout a porous membrane substrate so that the substrate is about 30 to about 80 volume percent porous;
covalently bonding a plurality of selective proteins to at least a portion of the functional groups; and
physically compressing the membrane substrate to produce an irreversibly compressed membrane substrate having a pore size of at least about 0.01 micron, a porosity of about 10 to about 50 volume percent, a density of about 1.25 to about 5.0 times the density of the membrane substrate before compressing and a thickness of about 0.2 to about 0.8 times the thickness of the membrane substrate before the compressing.
2 Assignments
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Accused Products
Abstract
A porous carrier containing an immobilized biologically active material is prepared in which a relatively large amount of an enzyme or an antibody has been immobilized in a manner that produces a low diffusion resistance toward reagents and products. The porous carrier is produced by fixing enzymes or antibodies within internal micropores of the carrier and mechanically compressing the carrier to a final thickness which is in the range of about 0.20 to 0.80 times the uncompressed carrier thickness. The compressed carrier may have a density about 1.25 to about 5.0 times the density of the carrier before compressing. Surprisingly, the compressed carrier exhibits less diffusion resistance to specific reagents and products than would an uncompressed carrier. A preferred porous carrier is a semi-permeable membrane made from synthetic polymers, such as polyvinylidine difluoride.
22 Citations
14 Claims
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1. A process for making a porous carrier containing an immobilized protein, which comprises:
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attaching a plurality of polar functional groups to an internal surface which defines a plurality of micropores distributed throughout a porous membrane substrate so that the substrate is about 30 to about 80 volume percent porous; covalently bonding a plurality of selective proteins to at least a portion of the functional groups; and physically compressing the membrane substrate to produce an irreversibly compressed membrane substrate having a pore size of at least about 0.01 micron, a porosity of about 10 to about 50 volume percent, a density of about 1.25 to about 5.0 times the density of the membrane substrate before compressing and a thickness of about 0.2 to about 0.8 times the thickness of the membrane substrate before the compressing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification