Bed material for cell culture
First Claim
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1. A method for culturing cells, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature to water within the range of 0°
- to 80°
C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface having hydrophobic properties and detaching and collecting the cultured cells from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, thereby changing the properties of said coated surface from hydrophobic to hydrophilic.
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Abstract
Disclosed is a bed material whereby cultured or grown cells are collected or detached from the material without a proteolysis enzyme or chemical material. The bed material comprises a support and a coating thereon, wherein the coating is formed from a polymer or copolymer which has a critical solution temperature to water within the range of 0° C. to 80° C.
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6 Claims
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1. A method for culturing cells, which comprises coating a support with a polymer or copolymer which has a lower or upper critical solution temperature to water within the range of 0°
- to 80°
C., culturing cells attached to the surface thereof at a higher temperature than said lower critical solution temperature or at a lower temperature than said upper critical solution temperature on said coated surface having hydrophobic properties and detaching and collecting the cultured cells from said coated surface by changing the temperature to a lower temperature than said lower critical solution temperature or at a higher temperature than said upper critical solution temperature, thereby changing the properties of said coated surface from hydrophobic to hydrophilic. - View Dependent Claims (2, 3, 4, 5, 6)
- to 80°
Specification
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Current AssigneeTokyo Women'S Medical College
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Original AssigneeKAO Corporation, Tokyo Women'S Medical College
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InventorsYamada, Noriko, Sakurai, Yasuhisa, Mamada, Akira, Kataoka, Kazunori, Okano, Teruo, Amiya, Takayuki
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Primary Examiner(s)Elliott, George C.
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Application NumberUS07/817,954Time in Patent Office763 DaysField of Search435/240.23, 435/240.243, 435/182US Class Current435/397CPC Class CodesC12N 2533/30 Synthetic polymers thermore...C12N 5/0068 General culture methods usi...
