Method and sensor electrode system for the electrochemical determination of an analyte or an oxidoreductase as well as the use of suitable compounds therefor
First Claim
1. A sensor electrode system for an electrochemical determination of the amount of an analyte in a liquid sample, comprisingan admixture of an oxidoreductase and a first reducible substance, wherein the reducible substance has the property that the reducible substance is reduced by said oxidoreductase to produce a reduced substance in a non-reversible reaction, and that, in turn, said reduced substance, when oxidized, produces a second reducible substance which is different from the first reducible substance;
- means for storing the admixture of the oxidoreductase and the first reducible substance;
means for contacting the admixture with the liquid sample which may contain the analyte;
contacting means for electrically contacting the admixture in contact with the sample with two physically separated electrically conductive leads;
said contacting means including an electrically conductive surface means for receiving electrons from the first reducible substance when the first reducible substance is reduced by said oxidoreductase to produce a reduced substance, and for oxidizing the reduced substance at the electrically conductive surface means to a second reducible substance which is different from the first reducible substance.
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Abstract
The subject matter of the invention is a method for the electrochemical determination of an analyte in the presence of an oxidoreductase and a reducible substance which transfers electrons which arise during the course of the determination reaction from the oxidoreductase onto an electrode and thus leads to a signal which is a measure for the analyte to be determined whereby the reducible substance is enzymatically reduced and oxidized at the electrode, which is characterized in that the substance which forms at the electrode by oxidation is different from the reducible substance used initially, as well as a corresponding sensor electrode system and the use of compounds suitable therefor. Finally new nitrosoaniline derivatives and a process for their production are also subject matter of the invention.
789 Citations
17 Claims
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1. A sensor electrode system for an electrochemical determination of the amount of an analyte in a liquid sample, comprising
an admixture of an oxidoreductase and a first reducible substance, wherein the reducible substance has the property that the reducible substance is reduced by said oxidoreductase to produce a reduced substance in a non-reversible reaction, and that, in turn, said reduced substance, when oxidized, produces a second reducible substance which is different from the first reducible substance; -
means for storing the admixture of the oxidoreductase and the first reducible substance; means for contacting the admixture with the liquid sample which may contain the analyte; contacting means for electrically contacting the admixture in contact with the sample with two physically separated electrically conductive leads; said contacting means including an electrically conductive surface means for receiving electrons from the first reducible substance when the first reducible substance is reduced by said oxidoreductase to produce a reduced substance, and for oxidizing the reduced substance at the electrically conductive surface means to a second reducible substance which is different from the first reducible substance. - View Dependent Claims (2, 3, 4, 5, 6, 8, 9, 11, 12, 13, 14, 16, 17)
- 4. The sensor electrode system of claim 1 wherein said reducible substance is a compound of the formula II
- space="preserve" listing-type="equation">HO--N=Y (II)
in which Y is a quinoid system which is, after reduction, electron-rich in the aromatic state.
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5. The sensor electrode system of claim 1 wherein said reducible substance is a nitrosoaniline derivative of the formula III ##STR27## in which R1 is hydrogen, halogen, alkoxy, or alkylthio, R2 is alkyl, and R3 is hydroxyalkyl;
- or
R2 and R3 are the same or different, and each may be dialkylaminoalkyl, hydroxyalkoxyalkyl, alkoxyalkyl or polyalkoxyalkyl wherein the alkyl residues may be unsubstituted or substituted with OH;
orR2 and R3 form an alkylene interrupted by sulphur or nitrogen, in which the nitrogen is substituted with an alkyl, hydroxyalkyl, hydroxyalkoxyalkyl, alkoxyhydroxyalkyl, dioxanylyl-alkyl, or polyalkoxyalkyl, and wherein the residue on the nitrogen is unsubstituted or substituted on the alkyl moiety by an OH;
orif R1 in the ortho position to NR2 R3, R2 and R1 also may form an alkylene, and R3 is an hydroxyalkyl, or, if the alkylene residue contains three carbon atoms, R3 may be an alkyl residue;
orR1 is not hydrogen, R2 and R3 are the same or different and each is a hydroxyalkyl;
ora salt of this derivative.
- or
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6. The sensor electrode system of claim 1 wherein said reducible substance is selected from the group consisting of
N-(2-hydroxyethyl)-N'"'"'-p-nitrosophenyl-piperazine, N,N-bis-(2-hydroxyethyl)-p-nitrosoaniline, o-methoxy-[N,N-bis-(2-hydroxyethyl)]-p-nitrosoaniline, p-hydroxynitrosobenzene, N-methyl-N'"'"'-(4-nitrosophenyl)-piperazine, p-quinone dioxime, N,N-dimethyl-p-nitrosoaniline, N,N-diethyl-p-nitrosoaniline, N-(4-nitrosophenyl)-morpholine, N-benzyl-N-(5'"'"'-carboxypentyl)-p-nitrosoaniline, N,N-dimethyl-4-nitroso-1-naphthylamine, N,N,3-trimethyl-4-nitrosoaniline, N-(2-hydroxyethyl)-5-nitrosoindoline, N,N-bis-(2-hydroxyethyl)-3-chloro-4-nitrosoaniline, -
8. The sensor electrode system of claim 1 wherein said reducible substance is N,N-bis-(2-hydroxyethyl)-p-nitrosoaniline or N-(2-hydroxyethyl)-N-(2-(2-hydroxyethoxy)ethyl)-4-nitrosoaniline.
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9. The sensor electrode system of claim 1 wherein said reducible substance is selected from the group consisting of
2,2'"'"'-[(3-fluoro-4-nitrosophenyl)imino]bis-ethanol, 2,2'"'"'-[(3-chloro-4-nitrosophenyl)imino]bis-ethanol, 2,2'"'"'[(3-methoxy-4-nitrosophenyl)imino]bis-ethanol, 2,2'"'"'[(3-methylmercapto-4-nitrosophenyl)imino]bis-ethanol, 2-[(2-hydroxyethoxy)ethyl-(4-nitrosophenyl)amino]ethanol, 2-[(2-methoxyethoxy)ethyl-(4-nitrosophenyl)amino]ethanol, 1-[N-(2-hydroxyethyl)-(4-nitrosoanilino)]-3-methoxy-2-propanol, 1-[N-(2-hydroxyethyl)-(4-nitrosoanilino)]-3-(2-hydroxyethoxy-2-propanol, 1-methyl-4-(4-nitrosophenyl)-piperazine, 4-(4-nitrosophenyl)-1-piperazine-ethanol, 5-nitroso-1-indoline ethanol, 1-methyl-6-nitroso-1,2,3,4-tetrahydroquinoline, 6-nitroso-3,4-dihydro-1(2H)quinoline ethanol and their salts. - 11. The sensor electrode system of claim 9 wherein said reducible substance is a compound of the formula I
- space="preserve" listing-type="equation">X--R (I)
in which R is an electron-rich aromatic residue, and X is NO or NHOH.
- space="preserve" listing-type="equation">HO--N═
Y (II)
- or
R2 and R3 are the same or different, and each may be dialkylaminoalkyl, hydroxyalkoxyalkyl, alkoxyalkyl or polyalkoxyalkyl wherein the alkyl residues may be unsubstituted or substituted with OH;
orR2 and R3 form an alkylene interrupted by sulphur or nitrogen, in which the nitrogen is substituted with an alkyl, hydroxyalkyl, hydroxyalkoxyalkyl, alkoxyhydroxyalkyl, dioxanylyl-alkyl, or polyalkoxyalkyl, and wherein the residue on the nitrogen is unsubstituted or substituted on the alkyl moiety by an OH;
orif R1 is in the ortho position to NR2 R3, R2 and R1 form an alkylene, and R3 is an hydroxyalkyl;
or, if the alkylene residue contains three carbon atoms, R3 may be an alkyl residue;
orif R1 is not hydrogen, R2 and R3 are the same or different and each is an hydroxyalkyl;
ora salt of this derivative.
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7. 4-dimethoxy-nitrosobenzene
N,N-bis-(2-methoxyethyl)-4-nitrosoaniline, 3-methoxy-4-nitrosophenol, N-(2-hydroxyethyl)-6-nitroso-1,2,3,4-tetrahydroquinoline, N,N-dimethyl-3-chloro-4-nitrosoaniline, N,N-bis-(2-hydroxyethyl)-3-fluoro-4-nitrosoaniline, N,N-bis-(2-hydroxyethyl)-3-methylthio-4-nitrosoaniline, N-(2-hydroxyethyl)-N-(2-(2-methoxyethoxy)-ethyl)-4-nitrosoaniline, N-(2-hydroxyethyl)-N-(3-methoxy-2-hydroxy-1-propyl)-4-nitrosoaniline, N-(2-hydroxyethyl)-N-(3-(2-hydroxyethoxy)-2-hydroxy-1-propyl)-4-nitrosoaniline, and N-(2-hydroxyethyl)-N-(2-(2-hydroxyethoxy)-ethyl)-4-nitrosoaniline.
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10. A sensor electrode system for an electrochemical determination of the amount of an oxidoreductase in a liquid sample, comprising
an admixture of a corresponding enzyme substrate and a first reducible substance, wherein the reducible substance has the property that the reducible substance is reduced by said oxidoreductase to produce a reduced substance in a non-reversible reaction, and that, in turn, said reduced substance, when oxidized, produces a second reducible substance which is different from the first reducible substance; -
means for storing the admixture of the enzyme substrate and the first reducible substance; means for contacting the admixture with the liquid sample which may contain the oxidoreductase; contacting means for electrically contacting the admixture in contact with the sample with two physically separated electrically conductive leads; said contacting means including an electrically conductive surface means for receiving electrons from the first reducible substance when the first reducible substance is reduced by said oxidoreductase to produce a reduced substance, and for oxidizing the reduced substance at the electrically conductive surface means to a second reducible substance which is different from the first reducible substance.
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15. 4-dimethoxy-nitrosobenzene
N,N-bis-(2-methoxyethyl)-4-nitrosoaniline, 3-methoxy-4-nitrosophenol, N-(2-hydroxyethyl)-6-nitroso-1,2,3,4-tetrahydroquinoline, N,N-dimethyl-3-chloro-4-nitrosoaniline, N,N-bis-(2-hydroxyethyl)-3-fluoro-4-nitrosoaniline, N,N-bis-(2-hydroxyethyl)-3-methylthio-4-nitrosoaniline, N-(2-hydroxyethyl)-N-(2-(2-methoxyethoxy)-ethyl)-4-nitrosoaniline, N-(2-hydroxyethyl)-N-(3-methoxy-2-hydroxy-1-propyl)-4-nitrosoaniline, N-(2-hydroxyethyl-N-(3-(2-hydroxyethoxy)-2-hydroxy-1-propyl)-4-nitrosoaniline, and N-(2-hydroxyethyl)-N-(2-(2-hydroxyethoxy)-ethyl)-4-nitrosoaniline.
Specification