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Surface-modified electochemical biosensor

  • US 5,286,364 A
  • Filed: 03/29/1991
  • Issued: 02/15/1994
  • Est. Priority Date: 06/08/1987
  • Status: Expired due to Term
First Claim
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1. A method of making a sensing element for use in a biosensor that amperometrically measures the concentration of an analyte in a biological liquid, said method comprising the following steps:

  • a) obtaining a first electrode having a sensing surface;

    b) immersing said sensing surface of said first electrode in a solution of monomer wherein said monomer is electropolymerizable into an electrically non-conducting polymer;

    c) flowing an electric current from a cathode, through said solution, to said first electrode, at a voltage and amperage sufficient to cause the monomer to be electropolymerized on the sensing surface of the first electrode;

    d) ceasing the current flow after the amperage of the current as measured at the first electrode declines to a relatively steady minimum, thereby yielding a sensing surface coated with an adherent layer of electrically non-conducting polymer;

    e) disposing with the polymeric coating on the sensing surface of the first electrode a chemical sensing agent that, when contacted by a specific analyte in a biological liquid, generates an electroactive molecule that can be detected amperometrically.

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