Method for assaying endotoxin in a whole blood sample or protein solution containing the same
First Claim
Patent Images
1. A method of assaying an endotoxin in a whole blood sample or a protein solution containing a whole blood sample, using a limulus amebocyte lysate component comprising the steps of:
- (a) obtaining a whole blood sample or a protein solution comprising a whole blood sample;
(b) treating said sample or solution, prior to contacting with the limulus amebocyte lysate component, by incubating said sample or solution in the presence of both nitric acid and a surfactant, separating the resulting precipitate, and collecting the supernatant, wherein the surfactant is selected from the group consisting of polyethylene glycol mono-p-iso-octylphenyl ether, polyethylene glycol mono-p-tert-octylphenoxypolyethoxyethanol, polyethylene glycol mono(nonylphenyl) ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan trioleate, n-octyl-α
-D-glucopyranoside, n-octyl-β
-D-glucopyranoside, n-nonyl-α
-D-glucopyranoside, n-nonyl-β
-D-glucopyranoside n-dodecyl-α
-D-glucopyranoside, n-dodecyl-β
-D-glucopyranoside, n-decyl-α
-D-glucopyranoside, n-decyl-β
-D-glucopyranoside, n-heptyl-α
-D-glucopyranoside, n-heptyl-β
-D-glucopyranoside, sodium dodecylsulfate and lithium dodecylsulfate; and
(c) assaying for endotoxin using the resulting treated sample or solution and the limulus amebocyte lysate component.
2 Assignments
0 Petitions
Accused Products
Abstract
A method of assaying an endotoxin using the limulus amebocyte lysate component which comprises pretreating whole blood with nitric acid and a surfactant selected from among polyoxyethylene ethers represented by the following formula: ##STR1## wherein n is an integer of from 8 to 40; or: ##STR2## polyoxyethylene sorbitans, n-alkylglucopyranosides, sodium dodecylsulfate and lithium dodecylsulfate, which enables an efficient and accuate assay of an endotoxin.
-
Citations
8 Claims
-
1. A method of assaying an endotoxin in a whole blood sample or a protein solution containing a whole blood sample, using a limulus amebocyte lysate component comprising the steps of:
-
(a) obtaining a whole blood sample or a protein solution comprising a whole blood sample; (b) treating said sample or solution, prior to contacting with the limulus amebocyte lysate component, by incubating said sample or solution in the presence of both nitric acid and a surfactant, separating the resulting precipitate, and collecting the supernatant, wherein the surfactant is selected from the group consisting of polyethylene glycol mono-p-iso-octylphenyl ether, polyethylene glycol mono-p-tert-octylphenoxypolyethoxyethanol, polyethylene glycol mono(nonylphenyl) ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan trioleate, n-octyl-α
-D-glucopyranoside, n-octyl-β
-D-glucopyranoside, n-nonyl-α
-D-glucopyranoside, n-nonyl-β
-D-glucopyranoside n-dodecyl-α
-D-glucopyranoside, n-dodecyl-β
-D-glucopyranoside, n-decyl-α
-D-glucopyranoside, n-decyl-β
-D-glucopyranoside, n-heptyl-α
-D-glucopyranoside, n-heptyl-β
-D-glucopyranoside, sodium dodecylsulfate and lithium dodecylsulfate; and(c) assaying for endotoxin using the resulting treated sample or solution and the limulus amebocyte lysate component. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
Specification