Consistent diagnostic test method using microwaves

• US 5,289,140 A
• Filed: 08/16/1991
• Issued: 02/22/1994
• Est. Priority Date: 08/16/1991
• Status: Expired due to Term

• Current Assignee: Dako (Agilent Technologies, Inc.)
• Original Assignee: Dako Japan Co., Ltd.
• Inventors: Vaboe, Bent B., Jorgenson, Finn
• Primary Examiner(s): MIS, DAVID C
• Application Number:
  US07/745,688
• Time in Patent Office: 921 Days
• Field of Search: 331/96, 331/56, 331/68, 331/74, 331/78, 219/10.55 B, 73/863.11
• US Class Current: 331/74
• CPC Class Codes: G01N 22/00 Investigating or analysing materials by the use of microwaves or radio waves, i.e. electromagnetic waves with a wavelength of one millimetre or more G01N3/00 - G01N17/00, G01N24/00 take precedence
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: H05B 6/705 using microwave tuning
• CPC Class Codes: H05B 6/806 for laboratory use

Abstract

Use of microwave equipment for fast food preparation and for speeding up chemical reactions e.g. in diagnostic testing is wellknown. However in all such uses the known equipment provides a very uneven heating effect, thereby limiting the value of the method. The present patent application describes an improved type of microwave equipment, in which an even effect is obtained by the use of a combination of microwaves of different wavelengths. The equipment according to the invention includes one or more microwave sources, controlled individually or jointly, emitting microwaves of a two or more wavelengths, e.g. in the form of distinct single wavelengths, White Noise, Square Waves.

Claims

1. A method for immunohistochemical testing comprising the steps of:

placing a sample to be tested on a sample slide;

treating the sample;

placing the sample and slide in a microwave cavity;

uniformly energizing the microwave cavity using microwave energy source means including at least three different wavelengths; and

energizing the cavity for a predetermined specified reaction time,

whereby samples are energized consistently with the proper level of microwave energy despite the variations in heating normally found in microwave heating cavities.

2. A method as defined in claim 1, wherein said step of uniformly energizing the microwave cavity includes the application of microwave energy from a substantially square wave source.

3. A method as defined in claim 1, wherein said step of uniformly energizing the microwave cavity includes the application of microwave energy from a substantially white noise source.

4. A method as defined in claim 1, including the additional step of removing the slide from the microwave cavity to review the visualization agents resulting from the process steps.

5. A method as defined in claim 1, wherein said step of uniformly energizing the microwave cavity includes applying the microwave energy to the cavity through a single radiating means coupled to the cavity.

6. A method for immunohistochemical testing comprising the steps of:

placing a sample to be tested in a sample slide;

treating the sample;

placing the sample and slide on a small shelf in a microwave cavity;

uniformly energizing the microwave cavity using microwave energy source means including at least three different wavelengths; and

energizing the cavity for a predetermined specified reaction time,

whereby samples are energized consistently with the proper level of microwave energy despite the variations in heating normally encountered throughout microwave heating cavities.

7. A method as defined in claim 6, wherein said step of uniformly energizing the microwave cavity includes the application of microwave energy from a substantially square wave source.

8. A method as defined in claim 6, wherein said step of uniformly energizing the microwave cavity includes the application of microwave energy from a substantially white noise source.

9. A method as defined in claim 6, including the additional step of removing the slide from the microwave cavity to review the visualization agents resulting from the process steps.

10. A method as defined in claim 6, wherein said step of uniformly energizing the microwave cavity includes applying the microwave energy to the cavity through a single radiating means coupled to the cavity.

11. A method as defined in claim 6, wherein the method includes the step of placing the sample on a microscopy glass slide.

12. Improved microwave equipment comprising:

a microwave source emitting waves of more than one frequency; and

means for supporting an immunohistochemical sample for uniform irradiation by said source.

13. Microwave equipment as defined in claim 12, further comprising a microwave cavity coupled to said microwave source.

14. Microwave equipment as defined in claim 13, wherein said microwave cavity is provided with a door, and a small internal shelf is provided behind said door.

15. Microwave equipment as defined in claim 14, wherein a glass slide carrying said sample is mounted on said shelf.