Cardioplegic compositions

• US 5,290,766 A
• Filed: 02/18/1992
• Issued: 03/01/1994
• Est. Priority Date: 02/18/1991
• Status: Expired due to Fees

• Current Assignee: National Heart Foundation of New Zealand
• Original Assignee: National Heart Foundation of New Zealand
• Inventors: Choong, Yee S.
• Primary Examiner(s): GRIFFIN, RONALD
• Application Number:
  US07/836,541
• Time in Patent Office: 742 Days
• Field of Search: 514/832, 514/833, 514/23, 435/1, 435/2, 128/897, 128/898, 604/49-52, 424/679
• US Class Current: 514/23
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/195 having an amino group
• CPC Class Codes: A61K 31/70 Carbohydrates; Sugars; Derivatives thereof sorbitol A61K31/047
• CPC Class Codes: A61K 31/7004 Monosaccharides having only carbon, hydrogen and oxygen atoms
• CPC Class Codes: A61K 33/00 Medicinal preparations containing inorganic active ingredients
• CPC Class Codes: A61K 33/14 Alkali metal chlorides; Alkaline earth metal chlorides

Abstract

An aqueous cardioplegic solution providing significantly improved cardiac function after a period of exposure to cardioplegia, contains NaCl in the range of 70-110 mM; KCl in the range of 10-16 mM; CaCl₂ in the range of 0.5-1.0 mM; MgCl₂ in the range of 8-16 mM; NaHCO₃ in the range of 10-20 mM; D-Glucose in the range of 5-15 mM; L-Aspartic acid in the range of 5-20 mM; and Lactobionic acid in the range of 0-15 mM.

Claims

1. A sterile aqueous cardioplegic solution comprising NaCl in the range of 70-110 mM;

KCl in the range of 10-16 mM;

CaCl₂ in the range of 0.5-1.0 mM;

MgCl₂ in the range of 8-16 mM;

NaHCO₃ in the range of 10-20 mM;

D-Glucose in the range of 5-15 mM;

L-Aspartic acid in the range of 5-20 mM; and

Lactobionic acid in the range of 0-15 mM.

2. A cardioplegic solution as claimed in claim 1, having a pH of about 7.5 and containing about 100 mM of NaCl;

about 10 mM of KCl;

about 0.8 mM of CaCl₂ ;

about 10 mM of MgCl₂ ;

about 10 mM of NaHCO₃ ;

about 10 mM of D-Glucose;

about 10 mM of L-Aspartic Acid; and

about 5 mM of Lactobionic Acid.

3. A method for creating a cardioplegic solution, in which the constituents are prepared and mixed as follows:

(a) the required amounts of aspartic and lactobionic acids are each dissolved in a small amount of distilled water and neutralized to pH 7.5;

(b) the cardioplegic solution is made by adding the various constituents in the sequence as listed here (1) NaCl, (2) KCl, (3) CaCl₂, (4) MgCl₂, (5) NaHCO₃, (6) D-Glucose, (7) L-Aspartic acid, and (8) Lactobionic acid to a large volume (about 70% total) of distilled water, mixing the solution after each addition;

(c) the balance of distilled water is added to the cardioplegic solution;

(d) the cardioplegic solution is bubbled with a gaseous mixture of 95% oxygen;

5% carbon dioxide for about 5 minutes; and

(e) the cardioplegic solution is passed through a cardioplegic filter before use.