Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds

• US 5,292,507 A
• Filed: 09/14/1992
• Issued: 03/08/1994
• Est. Priority Date: 08/01/1986
• Status: Expired due to Term

• Current Assignee: Eco Soil Systems, Inc.
• Original Assignee: Imperial Oil Limited (Exxon Mobil Corporation)
• Inventors: Charley, Robert
• Primary Examiner(s): Naff, David M.
• Application Number:
  US07/945,437
• Time in Patent Office: 540 Days
• Field of Search: 424/93, 435/41, 435/42, 435/243, 435/252.1, 435/252.2, 435/254, 435/260, 435/93 K, 435/93 N, 435/93 Q, 435/252.5, 435/253.3, 71/6, 71/7, 47/57.6, 47/58, 504/100
• US Class Current: 424/93.4
• CPC Class Codes: C05F 11/08 Organic fertilisers containing added bacterial cultures, mycelia or the like
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Abstract

Soluble, non-crosslinked polysaccharides are used to stabilize microorganisms for use as inoculants in agriculture. Preferably, the polysaccharide is alginate and plant seeds are inoculated. A solution of the polysaccharide and a suspension of the microorganism are mixed to form a composition containing about 0.005% to about 10% of the polysaccharide. The composition can be stored for one week or more before use and the composition may be dried.

Claims

1. A method for maintaining a microorganism comprising the steps of:

(i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth or yield of a plant;

(ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking;

(iii) preparing a suspension of said microorganism;

(iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4°

C.-30°

C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide; and

(v) storing the composition.

2. The method of claim 1 wherein said non-crosslinked polysaccharide is alginate.

3. The method of claim 2 wherein said alginate is present in said composition at a concentration of between 0.1-10%.

4. The method of claim 1 further comprising the step of drying said composition before said composition is stored.

5. The method of claim 4 wherein said drying is accomplished by air drying.

6. The method of any of claims 2, 3, 5, 1 or 4 wherein said composition is stored at a temperature of from about 4°

C. to about 30°

C. for a period of at least one week.

7. A method for inoculating a plant seed with a microorganism comprising the steps of:

(i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth of yield of a plant;

(ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking;

(iii) preparing a suspension of said microorganism;

(iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4°

C.-30°

C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide; and

(v) storing the composition;

(vi) contacting the plant seed with said composition; and

(vii) allowing said composition to inoculate the plant seed.

8. The method of claim 7 wherein said non-crosslinked polysaccharide is alginate.

9. The method of claim 8 wherein said alginate is present in said composition at a concentration of between 0.1-10%.

10. The method of claim 7 further comprising the step of drying said composition before said composition is stored.

11. The method of claim 10 wherein said drying is accomplished by air drying.

12. The method of any of claims 8, 9, 11, 7 or 10 wherein said composition is stored at a temperature of from about 4°

C. to about 30°

C. for a period of at least one week.