Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
First Claim
Patent Images
1. A method for maintaining a microorganism comprising the steps of:
- (i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth or yield of a plant;
(ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking;
(iii) preparing a suspension of said microorganism;
(iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4°
C.-30°
C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide; and
(v) storing the composition.
3 Assignments
0 Petitions
Accused Products
Abstract
Soluble, non-crosslinked polysaccharides are used to stabilize microorganisms for use as inoculants in agriculture. Preferably, the polysaccharide is alginate and plant seeds are inoculated. A solution of the polysaccharide and a suspension of the microorganism are mixed to form a composition containing about 0.005% to about 10% of the polysaccharide. The composition can be stored for one week or more before use and the composition may be dried.
-
Citations
12 Claims
-
1. A method for maintaining a microorganism comprising the steps of:
-
(i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth or yield of a plant; (ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking; (iii) preparing a suspension of said microorganism; (iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4°
C.-30°
C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide; and(v) storing the composition. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A method for inoculating a plant seed with a microorganism comprising the steps of:
-
(i) selecting a microorganism selected from the group consisting of Serratia, Arthrobacter, Azospirillium, Rhizobium, Bacillus, Pseudomonas fluorescens and Pseudomonas putida which has a beneficial effect on growth of yield of a plant; (ii) providing a solution comprising about 0.01% to about 20% of a substantially soluble, non-crosslinked polysaccharide having no more than about 10% of crosslinking; (iii) preparing a suspension of said microorganism; (iv) mixing the suspension of the microorganism with the polysaccharide solution to form a pourable composition which, at 4°
C.-30°
C., is a liquid, said composition comprising from about 0.005% to about 10% by weight of the non-crosslinked polysaccharide; and(v) storing the composition; (vi) contacting the plant seed with said composition; and (vii) allowing said composition to inoculate the plant seed. - View Dependent Claims (8, 9, 10, 11, 12)
-
Specification