Doped sol-gel glasses for obtaining chemical interactions

• US 5,292,801 A
• Filed: 08/31/1992
• Issued: 03/08/1994
• Est. Priority Date: 01/23/1990
• Status: Expired due to Term

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Inventors: Zusman, Riyka, Avnir, David, Braun, Sergei, Ottolenghi, Michael
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US07/937,258
• Time in Patent Office: 554 Days
• Field of Search: 525/54.1, 514/2, 514/21, 530/402, 530/403, 530/405, 530/408, 530/409, 530/811, 435/174, 435/175, 501/12, 501/32
• US Class Current: 525/54.1
• CPC Class Codes: B01D 15/422 Displacement mode
• CPC Class Codes: B01J 20/283 based on silica
• CPC Class Codes: B01J 20/291 Gel sorbents
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: C03C 4/0007 for biologically-compatible glass
• CPC Class Codes: C12N 11/14 Enzymes or microbial cells immobilised on or in an inorganic carrier
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/92 Construction of the plate
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/5436 with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: G01N 33/6869 Interleukin
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y02P 20/50 Improvements relating to the production of bulk chemicals
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10T 428/2989 Microcapsule with solid core [includes liposome]
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Abstract

A method is proposed of obtaining a chemical interaction between at least one reagent trapped in sol-gel glass by doping it with the reagent, and diffusible solutes or components in an adjacent liquid or gas phase. The reagents, the solutes or the components can be any organic or inorganic compounds or materials of biological origin including enzymes. The doped sol-gel glass in various forms may be useful as analytical test, chromatographic medium, sensor, catalyst or biocatalyst, electrode or enzyme electrode, or other detection device.

Claims

1. A process for the preparation of a reactive sol-gel glass, comprising polymerizing at least one monomer of the formula M(R)_n (P)_m and selected from the group consisting of metal alkoxides, semi-metal alkoxides, metal esters and semi-metal esters, wherein M is a metallic or semi-metallic element, R is a hydrolyzable substituent, n is an integer of 1 to 6, P is a non-polymerizable substituent and m is an integer of 0 to 6, and optionally an organic monomer, under acidic, neutral or basic conditions and in the presence of a dopant to form a porous xerogel containing the dopant trapped therein, said polymerization including a gelling step conducted at not greater than room temperature and a drying step conducted at not greater than 45°

C., said dopant being selected from the group consisting of organic compounds, stable organic radicals, organometallic compounds, inorganic compounds and molecules of biological origin, the dopant being reactive after preparation of the xerogel.

2. A process as defined by claim 1, wherein M is at least one metal selected from the group consisting of Si, Al, Ti and Pb, R is at least one substituent selected from the group consisting of alkyl, aryl, alkoxy and aryloxy groups, and n is 2, 3 or 4.

3. A process as defined by claim 1, wherein the sol-gel glass is formed from at least two monomers of the formula M(R)_n.

4. A process as defined by claim 1, wherein dopant molecules are trapped in the sol-gel glass.

5. A process as defined by claim 1, wherein the xerogel is transparent.