×

Real-time monitoring of oxidative products from in vitro cell-biomaterial interaction using chemiluminescence

  • US 5,294,541 A
  • Filed: 07/13/1992
  • Issued: 03/15/1994
  • Est. Priority Date: 09/21/1989
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for analyzing in real time in vitro interactions between biological cells and biomaterial proposed for implantation which comprises:

  • introducing biological cells and biomaterial proposed for implantation into an environment for interaction, wherein said environment includes chemiluminescent probes which emit chemiluminescent light in the presence of oxidative products produced by said cells;

    monitoring over real time the amount of chemiluminescent light produced by oxidative products of said cells in the presence of said biomaterial as a first measurement;

    introducing a biological activator into said environment, wherein said biological activator causes the production of oxidative products;

    monitoring over real time the amount of chemiluminescent light produced by oxidative products of said cells as a second measurement;

    repeating the above steps under the same conditions except without the introduction of said biomaterial; and

    measuring the amount of said oxidative products produced by the interaction between said biological cells and said biomaterial in said environment over real time based on a comparison of each of the first chemiluminescence measurements, respectively, and each of the second chemiluminescence measurements, respectively.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×