Method and kit for detecting explosives

• US 5,296,380 A
• Filed: 09/03/1992
• Issued: 03/22/1994
• Est. Priority Date: 09/03/1992
• Status: Expired due to Fees

• Current Assignee: Israel Institute for Biological Research
• Original Assignee: Israel Institute for Biological Research
• Inventors: Margalit, Yair
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): EDWARDS, LAURA ESTELLE
• Application Number:
  US07/939,828
• Time in Patent Office: 565 Days
• Field of Search: 73/23.2, 436/106, 436/96, 436/110, 436/111, 436/124, 422/61
• US Class Current: 436/106
• CPC Class Codes: F41H 11/134 Chemical systems, e.g. with detection by vapour analysis
• CPC Class Codes: F42B 35/00 Testing or checking of ammunition apparatus for measuring the energy of projectiles G01L5/14
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/227 Explosives, e.g. combustive properties thereof
• CPC Class Codes: Y10T 436/17 Nitrogen containing
• CPC Class Codes: Y10T 436/173076 Nitrite or nitrate
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium
• CPC Class Codes: Y10T 436/19 Halogen containing

Abstract

In a color reaction method for detecting particular classes of explosives, a sample from a suspect source is subjected serially to the indicated reagents in the liquid phase, the reagents after the first being applied only if no color reaction has been obtained in the preceding test. The second and third reagents only are applied in presence of the preceding reagent(s). The first reagent is an alkaline solution of a diazotizable aminoaromatic azo-dye precursor, which detects nitroaromatic explosives. The second reagent is a strongly acidic reagent containing a nitrate to nitrite ion reducing agent and a diazo-coupler, which detects organic nitrates and nitramines. The third reagent is zinc powder suspended in a liquid, which detects inorganic nitrates. The fourth reagent is an aniline salt in a homogeneous strongly acidic solution including a water-miscible organic solvent, which detects chlorates and bromates. The invention includes also a test kit for use in the method.

Claims

1. A method for detecting explosives selected from nitroaromatics, organic nitrates, nitramines, inorganic nitrates, chlorates and bromates, which method comprises providing a sample from a suspect source, and subjecting said sample to the steps of:

contacting the sample with a first reagent, which is an alkaline solution of a diazotizable aminoaromatic azo-dye precursor, whereby the presence of a nitroaromatic explosive affords a distinct coloration; and

in the absence of such coloration,

contacting the sample, which is already in contact with said first reagent, with a second reagent containing a nitrate to nitrite ion reducing agent and a diazo-coupler which gives a colored product on reaction with a diazonium compound formed by reaction of the diazotizable aminoaromatic azo-dye precursor with nitrite ion, said second reagent being sufficiently strongly acidic in that it neutralizes and makes acidic a mixture thereof with an equal volume of said first reagent in contact with said sample, whereby the presence of an organic nitrate or nitramine explosive affords a distinct coloration; and

in the absence of such coloration,

contacting the sample, which is already in contact with said first and second reagents, with zinc powder suspended in a liquid, whereby the presence of inorganic nitrate affords a distinct coloration; and

in the absence of a distinct coloration in all of the preceding steps, providing a second sample from said source, and;

contacting said second sample with an aniline salt in a homogeneous strongly acidic solution including at least one water-miscible organic solvent, whereby the presence of chlorate or bromate affords a distinct coloration.

2. A method according to claim 1, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

3. A method according to claim 2, wherein said diazocoupler contains an aminonaphthyl moiety and said second reagent comprises aqueous oxyacid.

4. A method according to claim 2, wherein said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

5. A method according to claim 2, wherein either said first reagent, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

6. A method according to claim 2, wherein said diazo-coupler is N-(1-naphthyl) ethylenediamine, said second reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt.

7. A method according to claim 2, wherein said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide.

8. A method according to claim 2, wherein either said first reagent, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

9. A method according to claim 1, wherein said diazo-coupler contains an aminonaphthyl moiety and said second reagent comprises aqueous oxyacid.

10. A method according to claim 1, wherein said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

11. A method according to claim 1, wherein either said first reagent, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

12. A method according to claim 1, wherein:

said diazo-coupler contains an aminonaphthyl moiety and said second reagent comprises aqueous oxyacid;

said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent; and

either said first reagent, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

13. A method according to claim 1, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

14. A method according to claim 1, wherein said diazo-coupler is N-(1-naphthyl)ethylenediamine, said second reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt.

15. A method according to claim 1, wherein said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide.

16. A method according to claim 1, wherein either said first reagent, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

17. A method according to claim 1, wherein:

said diazo-coupler is N-(1-naphthyl)ethylenediamine, said second reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt;

said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide; and

either said first reagent, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

18. A method according to claim 4, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

20. A test kit according to claim 18, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

21. A test kit according to claim 20, wherein said diazo-coupler contains an aminonaphthyl moiety and said reagent comprises aqueous oxyacid.

22. A test kit according to claim 20, wherein said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

23. A test kit according to claim 20, wherein either said alkaline solution, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

24. A test kit according to claim 20, wherein said diazo-coupler is N-(1-naphthyl)ethylenediamine, said reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt.

25. A test kit according to claim 20, wherein said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide.

26. A test kit according to claim 20, wherein either said alkaline solution, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

19. A test kit for use in a method for detecting explosives selected from nitroaromatics, organic nitrates, nitramines, inorganic nitrates, chlorates and bromates, which kit comprises the following components:

absorbent means for sampling a suspected source for explosives wherein said suspected source includes a substance, a surface of an inanimate object and an exterior periphery of a human;

an alkaline solution of a diazotizable aminoaromatic azo-dye precursor, wherein said solution is contained in dispensing means;

a reagent which is a solution containing a nitrate to nitrite ion reducing agent and a diazo-coupler which gives a colored product on reaction with a diazonium compound formed by reaction of said diazotizable aminoaromatic azo-dye precursor with nitrite ion, said solution being sufficiently strongly acidic in that it neutralizes and makes acidic a mixture thereof with an equal volume of said alkaline solution, wherein said reagent is contained in dispensing means;

an admixture of zinc powder with liquid which on shaking gives a suspension of said zinc powder therein, wherein said admixture is contained in dispensing means; and

an aniline salt in a homogeneous strongly acidic solution including at least one water-miscible organic solvent, wherein said strongly acid solution is contained in dispensing means.

27. A test kit according to claim 19, wherein said diazo-coupler contains an aminonaphthyl moiety and said reagent comprises aqueous oxyacid.

28. A test kit according to claim 19, wherein said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

29. A test kit according to claim 19, wherein either said alkaline solution, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

30. A test kit according to claim 19, wherein:

said diazo-coupler contains an aminonaphthyl moiety and said reagent comprises aqueous oxyacid;

said strongly acidic solution comprises an oxyacid and contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent; and

either said alkaline solution, or said liquid, or both, contains also at least one water-miscible alcohol and at least one other water-miscible organic solvent.

31. A test kit according to claim 30, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

32. A test kit according to claim 19, wherein said diazo-coupler is N-(1-naphthyl)ethylenediamine, said reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt.

33. A test kit according to claim 19, wherein said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide.

34. A test kit according to claim 19, wherein either said alkaline solution, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

35. A test kit according to claim 31, wherein:

said diazo-coupler is N-(1-naphthyl)ethylenediamine, said reagent comprises aqueous phosphoric acid and said reducing agent comprises hydrazine and a thiosulfate salt;

said strongly acidic solution comprises sulfuric acid and contains also ethanol and dimethyl sulfoxide; and

either said alkaline solution, or said liquid, or both, contains also dimethyl sulfoxide and at least one alcohol selected from methyl alcohol and isopropyl alcohol.

36. A test kit according to claim 35, wherein said diazotizable aminoaromatic azo-dye precursor is sulfanilamide.

37. A test kit according to claim 19, wherein said alkaline solution, reagent, admixture and strongly acid solution are contained in separate sealed breakable ampoules which are enclosed in plastic tubes, said tubes being adapted for dropwise dispensing of the contents of the ampoules when these are broken, thus releasing said contents into said tubes.

38. A test kit according to claim 37, wherein at least said ampoules containing said alkaline solution and said reagent contain also an inert gas to minimize deterioration of their contents.