Closure device for sealing reagent containers in an automatic pipetting system

• US 5,297,599 A
• Filed: 03/10/1992
• Issued: 03/29/1994
• Est. Priority Date: 03/19/1991
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Bucheli, Rudolf
• Primary Examiner(s): JACYNA, J CASIMER
• Application Number:
  US07/848,766
• Time in Patent Office: 749 Days
• Field of Search: 73/863.85, 73/863.86, 73/864.01, 73/864.14, 73/864.74, 73/684.86, 222/80, 222/81, 222/85, 222/86, 141/329, 141/330, 141/18, 141/21, 141/25-28, 141/130, 215/247-250, 215/276, 215/307, 215/354, 215/364, 215/DIG. 3, 604/403, 604/411, 604/415
• US Class Current: 141/329
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10S 215/03 Medical

Abstract

A closure or cap for a reagent container for use in an analysis system in which an automatic pipetting device is used for transferring small quantities of reagent from the reagent container to reaction cells. In order to simplify the process of handling the reagent container in an automatic analysis system, the closure is characterized in that it is formed in one piece from a plastic and it has a cylindrical side wall and an annular top wall adjacent the side wall and merging into a conical wall which extends inwards from the inner circle of the top wall and ends in a piercable tip situated on the axis of symmetry of the cylindrical wall.

Claims

1. A cap member for a reagent container for use in an analysis system with an automatic pipetting device having a pipetting needle for transferring small quantities of at least one reagent from the reagent container to a reaction cell, the cap comprising

a) an outer cylindrical side wall having an inner and an outer surface and having an axis of symmetry parallel to the side wall;

b) an annular top wall adjacent to the side wall and having a circular aperture; and

c) an inner conical wall with an inner and outer surface adjacent to the top wall tapering inwardly from the aperture in the top wall and away from the side wall along the axis of symmetry, and ending at a tip configured and proportioned to be piercable by the pipetting needle,

the cap member being formed as a single piece of plastic material wherein the inner conical wall has a first and second part, the first part forms a first angle with the axis of symmetry and the second part ending in the tip forms a second angle with the axis of symmetry, the second angle being greater than the first angle, wherein the first angle is about 5°

to about 30° and

the second angle is about 20°

to about 60°

with respect to the axis of symmetry.

2. The cap according to claim 1, further comprising a retaining means.

3. The cap according to claim 2, wherein the retaining means comprises an annular screw thread along the inner surface of the side wall.

4. The cap according to claim 1, wherein the first angle is about 10°

and the second angle is about 30°

.

5. The cap according to claim 1, further comprising a sealing means positioned and configured to cover the circular aperture of the top wall.

6. The cap according to claim 5, wherein the sealing means comprises a metal foil.

7. The cap according to claim 1, further comprising a sealing lip projecting from the top wall and positioned between the side wall and the inner conical wall at a position from the side wall to permit a portion of the container to fit between the lip and the side wall in a sealed manner.

8. A closure device for a reagent container for use in an analysis system with an automatic pipetting device having a pipetting needle for transferring small quantities of at least one reagent from the reagent container to a reaction cell, the closure device comprising

a) a cap member comprising an outer cylindrical side wall having an inner and an outer surface and having an axis of symmetry parallel to the side wall, an annular top wall adjacent to the side wall and having circular aperture, an inner conical wall with an inner and outer surface adjacent to the top wall tapering inwardly from the aperture in the top wall and away from the side wall along the axis of symmetry, and ending at a tip configured and proportioned to be piercable by the pipetting needle, the cap member being formed as a single piece of plastic material, and

b) a first sealing means for forming a seal between the cap and the container wherein the inner conical wall of the cap has at least a first and second part, the first forms a first angle with the axis of symmetry and the second part ending in the tip forms a second angle with the axis of symmetry, the second angle being greater than the first angle, wherein the first angle is about 5°

to about 30° and

the second angle is about 20°

to about 60°

with respect to the axis of symmetry.

9. The closure device according to claim 8, wherein the cap further comprises an annular screw thread along the inner surface of the side wall.

10. The closure device according to claim 8, wherein the sealing means comprises a plug configured and proportioned to be insertable between the cap and the container, having a central orifice with an inner wall configured to abut the outer surface of the conical wall in a sealing manner.

11. The closure device according to claim 8, wherein the first angle is about 10°

and the second angle is about 30°

.

12. The closure device according to claim 11, further comprising a second sealing means positioned and configured to cover the circular aperture of the top wall.

13. The closure device according to claim 12, wherein the second sealing means is a metal foil.