Chemically wired fructose dehydrogenase electrodes
First Claim
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1. An enzyme electrode for measuring D-fructose concentration is liquids comprising:
- (a) a fructose dehydrogenase enzyme containing pyrrolo quinoline quinone cofactor;
(b) a conductive polymer comprising a water soluble polyvinylpyridine complex of Os(bipyridine)2 Cl; and
(c) an electron collector, said conductive polymer being chemically boned to said enzyme and adsorbed on said electron collector, said enzyme electrode being devoid of electron mediator save for said conductive polymer.
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Abstract
An enzyme electrode is disclosed for use in voltammetric systems wherein a PQQ dehydrogenase enzyme is chemically bonded to a water soluble polyvinylpyridine osmium bipyridine polymer. The enzyme and polymer are adsorbed onto the surface of the electrode with the polymer providing direct electron transfer from enzyme to electrode and vice-versa without the aid of additional mediators or electron shuttles added to the system.
381 Citations
8 Claims
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1. An enzyme electrode for measuring D-fructose concentration is liquids comprising:
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(a) a fructose dehydrogenase enzyme containing pyrrolo quinoline quinone cofactor; (b) a conductive polymer comprising a water soluble polyvinylpyridine complex of Os(bipyridine)2 Cl; and (c) an electron collector, said conductive polymer being chemically boned to said enzyme and adsorbed on said electron collector, said enzyme electrode being devoid of electron mediator save for said conductive polymer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification