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Pipette calibration system

  • US 5,298,978 A
  • Filed: 02/28/1992
  • Issued: 03/29/1994
  • Est. Priority Date: 02/28/1992
  • Status: Expired due to Term
First Claim
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1. A method for determining the volume of a vessel using a photometer, comprising the steps of:

  • a) positioning a container in a photometer, said container holding a reference solution having maximum absorbance at a first wavelength;

    b) measuring the absorbance of said container at said first wavelength and at a second wavelength;

    c) calculating the optical pathlength of said container at said second wavelength from the measured absorbances at said first and second wavelengths;

    d) introducing an aliquot of a sample solution into said container from a vessel to be calibrated, said sample solution having maximum absorbance at said second wavelength, and mixing said sample solution with said reference solution to form a mixture;

    e) measuring the absorbance of said mixture in said container at said second wavelength; and

    f) calculating the volume of the aliquot from the absorbance of said mixture and the optical pathlength of said container, the volume of said aliquot representing the volume of the vessel.

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