Two- and three-dimensional autoradiographic imaging utilizing charge coupled devices
First Claim
1. An apparatus for producing a three-dimensional autoradiographical image of a tissue sample containing a radioactively tagged substance comprising:
- a) a solid state radiation detecting means for detecting said radioactively tagged substance in said tissue sample;
b) means for sectioning said tissue sample to provide a section of tissue sample having a first surface and thereby providing a further exposed surface on said tissue sample;
c) means for processing information generated by said solid state radiation detecting means into a plurality of two-dimensional images and for further processing said plurality of two-dimensional images into said three-dimensional image of said tissue sample;
d) manipulating means for positioning said section of said tissue sample in a predetermined location;
e) control means for controlling said solid state radiation detecting means, said means for processing information generated by said solid state radiation detecting means, said means for sectioning and said manipulating means to automatically analyze said tissue sample and produce said three-dimensional image of said tissue sample containing said radioactively tagged substance.
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Abstract
A two- and three-dimensional autoradiographical imaging system is provided which includes a charge coupled device for detecting the emission of radioactively labeled substances from materials such as tissue samples, brains of humans or animals, or substances used in electrophoresis applications. In a first aspect, a radioactively labeled substance is included in a tissue sample. The tissue sample is sequentially imaged by a charge coupled device and a sectioning tool such as a microtome to produce a plurality of two-dimensional images. A three-dimensional image of the tissue sample is generated by further processing of the plurality of two-dimensional images derived from the charge coupled device. In a further aspect of the invention, a charge coupled device is utilized to provide realtime imaging of metabolic or physiological parameters involved in brain activity. A charge coupled device is positioned adjacent a portion of brain tissue desired to be examined, the brain tissue having a radioactively labeled substance therein for detection by the charge coupled device. A two-dimensional realtime image is produced using the charge coupled device for use in clinical or behavioral studies.
39 Citations
26 Claims
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1. An apparatus for producing a three-dimensional autoradiographical image of a tissue sample containing a radioactively tagged substance comprising:
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a) a solid state radiation detecting means for detecting said radioactively tagged substance in said tissue sample; b) means for sectioning said tissue sample to provide a section of tissue sample having a first surface and thereby providing a further exposed surface on said tissue sample; c) means for processing information generated by said solid state radiation detecting means into a plurality of two-dimensional images and for further processing said plurality of two-dimensional images into said three-dimensional image of said tissue sample; d) manipulating means for positioning said section of said tissue sample in a predetermined location; e) control means for controlling said solid state radiation detecting means, said means for processing information generated by said solid state radiation detecting means, said means for sectioning and said manipulating means to automatically analyze said tissue sample and produce said three-dimensional image of said tissue sample containing said radioactively tagged substance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. The method of producing a three-dimensional autoradiographical image of a tissue sample containing a radioactively tagged substance comprising the steps of:
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a) providing a tissue sample containing a radioactively tagged substance; b) providing a solid state radiation detecting means; c) detecting said radioactively tagged substance on a top surface of said tissue sample using said solid state radiation detecting means and producing a first two-dimensional image thereof; d) removing a section of said tissue sample and exposing a further surface of said tissue sample; e) detecting said radioactively tagged substance in said further surface using said solid state radiation detecting means and producing a second two-dimensional image thereof; f) repeating steps (d) and (e) to produce further surfaces of said tissue sample so as to produce a plurality of two-dimensional images of each of said further surfaces; and g) processing each of said two-dimensional images to produce a three-dimensional image of said tissue sample containing said radioactively tagged substance. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. The method of producing a three-dimensional autoradiographical image of a tissue sample containing a radioactively tagged substance comprising the steps of:
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a) providing a tissue sample containing a radioactively tagged substance; b) providing a solid state radiation detecting means; c) removing a section of said tissue sample and exposing a further surface of said tissue sample; d) detecting said radioactively tagged substance in said section of said tissue sample using said solid state radiation detecting means and producing a two-dimensional image thereof; e) repeating steps of (c) and (d) to produce a plurality of said sections of said tissue sample and a plurality of two-dimensional images from said plurality of said sections; and f) processing said plurality of two-dimensional images to produce the three-dimensional image of said tissue sample containing said radioactively tagged substance. - View Dependent Claims (22, 23, 24, 25, 26)
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Specification