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Method for determination of components

  • US 5,302,513 A
  • Filed: 07/09/1991
  • Issued: 04/12/1994
  • Est. Priority Date: 06/29/1988
  • Status: Expired due to Fees
First Claim
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1. In a method for determination of amylase activity in a biological sample, which comprises the steps of:

  • (1) mixing the sample with NAD(P), ATP, glucokinase, glucose-6-P-dehydrogenase, maltose phosphorylase and phosphate to form a first reaction solution;

    (2) subjecting the first reaction solution to reaction with maltopentose to form a second reaction solution; and

    (3) measuring the absorption of the second reaction solution at 340 nm, the improvement comprising;

    carrying out the reaction of step (1) at 25°

    to 50°

    C. at pH 6 to 9 in the presence of glutathione reductase and glutathione of oxidation type; and

    carrying out the reaction of step (2) at 25°

    to 50°

    C. at pH 6 to 9 in the presence of dithiothreitol, γ

    -glutamyl transpeptidase and glycylglycine.

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