Evoked 23NA MR imaging of sodium currents in the brain

• US 5,303,705 A
• Filed: 05/01/1992
• Issued: 04/19/1994
• Est. Priority Date: 05/01/1992
• Status: Expired due to Fees

• Current Assignee: Nenov, Valeriy I.
• Original Assignee: Nenov, Valeriy I.
• Inventors: Nenov, Valeriy I.
• Primary Examiner(s): Smith, Ruth S.
• Application Number:
  US07/877,950
• Time in Patent Office: 718 Days
• Field of Search: 128/653.2, 324/307, 324/309
• US Class Current: 600/410
• CPC Class Codes: G01R 33/4806 Functional imaging of brain activation
• CPC Class Codes: G01R 33/561 by reduction of the scanning time, i.e. fast acquiring systems, e.g. using echo-planar pulse sequences
• CPC Class Codes: G01R 33/563 of moving material, e.g. flow contrast angiography
• CPC Class Codes: G01R 33/567 gated by physiological signals , i.e. synchronization of acquired MR data with periodical motion of an object of interest, e.g. monitoring or triggering system for cardiac or respiratory gating

Abstract

A system and method of Evoked Sodium Magnetic Resonance Imaging (ESMRI) for three-dimensional localization and measurement of sodium currents in the brain during neuronal activity is disclosed. The system measures neuronal activity in response to a stimulus. The sodium MRI measures changes in the sodium concentration due to the influx of sodium into neurons during the generation of synaptic and action potentials. The system measures primarily extracellular sodium which is distinguished from intracellular sodium on the basis of different spin-spin relaxation rates (T2) for these two compartments. Repeated measurements of sodium concentrations are used to produce and display the changes in concentration cinematically. The technique of the present invention is noninvasive and produces three-dimensional brain images with sufficient spatial and temporal resolution to allow three-dimensional visualization of the sequence of neuronal activation in the brain during processing of sensory, motor, cognitive and other tasks.

Claims

1. A method for measuring neuronal activity in a subject'"'"'s brain, said method comprising the steps of:

a) causing said subject'"'"'s brain to generate neuronal activity;

b) imaging sodium concentration in a portion of said brain using a nuclear magnetic resonance imaging (MRI) apparatus, said apparatus being adapted to produce sodium magnetic resonance images, wherein said magnetic resonance image is primarily a measure of the momentary extracellular sodium concentration in said portion of the subject'"'"'s brain;

c) repeating said step of imaging a plurality of times after said step of causing said subject to produce neuronal activity, wherein, a plurality of said images are produced following the generation of said neuronal activity;

d) displaying said plurality of magnetic resonance images; and

e) measuring neuronal activity from the location of changes in the sodium concentration between successive magnetic resonance images which correlates with the location of said neuronal activity in the brain.

2. The method of claim 1 wherein said step of imaging is repeated at intervals of time less than 50 msec apart.

3. The method of claim 2 wherein only the T2 nuclear magnetic resonance echo from the sodium atoms is measured.

4. The method of claim 1 further comprising the step of using a radio frequency pulse to drive the atoms of said sodium atoms back to equilibrium after said step of imaging is performed.

5. The method of claim 1, further comprising the step of;

repeating steps a-c a plurality of times to produce a plurality of trials each consisting of a temporal sequence of images; and

averaging all of the corresponding images in the plurality of trails.

6. The method of claim 1 wherein step c further comprises the step of determining the differences between successive images produced by step c, wherein said step of displaying displays only the changes in said sodium concentration in the brain.

7. The method of claim 1 wherein the step of displaying further comprises the step of displaying said plurality of magnetic resonance images superimposed over a proton MRI image of said brain.

8. The method of claim 1 wherein said step of displaying further comprises the step of displaying said images cinematically.

9. The method of claim 1 wherein said step of imaging said brain further comprises the step of creating a three-dimensional image of said brain.

10. The method of claim 1 herein said step of causing subject to produce neuronal activity comprises the step of presenting a visual stimulation to said subject.

11. The method of claim 1 wherein said step of causing said subject to produce neuronal activity comprises the step of presenting an auditory stimulation to said subject.

12. A system for measuring neuronal activity in a subject'"'"'s brain, said system comprising:

means for causing said subject'"'"'s brain to produce neuronal activity;

nuclear magnetic resonance imaging (MRI) means, for producing sodium magnetic resonance images, wherein said magnetic resonance images are primarily a measure of the momentary extracellular sodium concentration in said portion of the subject'"'"'s brain;

host computer for controlling said MRI means to generate a temporal sequence of said images;

means for displaying said plurality of MRI images; and

means for measuring neuronal activity from the location of changes in the sodium concentration between successive images which correlates with the location of neuronal activity produced by said means for said subject to produce neuronal activity.

13. The system of claim 12 wherein said host computer comprises a means for generating a pulse sequence controlling the generation of RF pulses.

14. The system of claim 13 further comprising an RF transceiver coupled to said host computer for transmitting and receiving said RF pulses, and wherein said means for generating a pulse sequence causes said RF pulses to be transmitted and received at intervals less than 50 msec apart.

15. The system of claim 14 wherein said means for generating a pulse sequence includes a means for generating a radio frequency pulse to drive the nuclei of said sodium atoms back to equilibrium after each of said images are produced.

16. The system of claim 15 wherein said means for generating a radio frequency pulse comprises a means for generating a Driven Equilibrium Fourier Transform (DEFT).

17. The system of claim 12 wherein said MRI means produces only T2 nuclear magnetic resonance echo images.

18. The system of claim 13 wherein said means for displaying displays only the changes in said sodium concentration in the brain between successive images.

19. The system of claim 13 wherein the means for displaying further comprises means for displaying said plurality of magnetic resonance images superimposed over a proton MRI image of said brain.

20. The system of claim 13 wherein said means for displaying displays said plurality of images in rapid sequence cinematically.

21. The system of claim 13 wherein said MRI means includes means for generating a three-dimensional image of a portion of said brain.

22. The system of claim 12 wherein said means for causing said subject'"'"'s brain to produce neuronal activity comprises means for presenting a visual stimulation to said subject.

23. A system for measuring neuronal activity in a subject'"'"'s brain, said system comprising:

means for stimulating said subject'"'"'s brain to produce neuronal activity;

nuclear magnetic resonance imaging (MRI) means for producing sodium magnetic resonance images, wherein said magnetic resonance images are primarily a measure of the momentary extracellular sodium concentration in said portion of the subject'"'"'s brain;

host computer means for controlling said MRI means to generate a plurality of said images, each of said images comprising the T2 echo from said sodium;

means for displaying said plurality of MRI images; and

means for measuring neuronal activity from the location of changes in the sodium concentration between successive images which correlates with the location of neuronal activity produced by said means for stimulating said subject to produce neuronal activity.