Method of eradicating infectious biological contaminants

• US 5,304,113 A
• Filed: 11/02/1990
• Issued: 04/19/1994
• Est. Priority Date: 11/21/1986
• Status: Expired due to Term

• Current Assignee: Medical College of Wisconsin Incorporated
• Original Assignee: MCW Research Foundation Inc (Medical College of Wisconsin Incorporated)
• Inventors: Sieber, Fritz, Smith, Orla M.
• Primary Examiner(s): Hafer, Robert A.
• Assistant Examiner(s): RIMELL, SAMUEL G
• Application Number:
  US07/608,458
• Time in Patent Office: 1,264 Days
• Field of Search: 604/4, 604/5, 604/6, 604/19, 604/27, 604/48, 604/49, 604/20
• US Class Current: 604/6.08
• CPC Class Codes: A61K 31/505 Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
• CPC Class Codes: A61K 31/515 Barbituric acids; Derivatives thereof, e.g. sodium pentobarbital
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/08 Radiation
• CPC Class Codes: A61M 1/3623 Means for actively controlling temperature of blood
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3686 by removing photoactive agents after irradiation
• CPC Class Codes: C12N 2710/16663 by chemical treatment
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00

Abstract

A method for externally eradicating by inactivation infectious pathogenic contaminants in body tissues, such as blood, so that it can be introduced into an animal comprises admixing the body tissues with an effective, non-toxic, amount of a photoactive agent which selectively binds to the contaminant, passing the resulting mixture through an irradiation chamber, and then irradiating the resulting mixture with an effective level of visible light for an effective period of time to eradicate the contaminants.

Claims

1. A method for eradicating infectious pathogenic biological contaminants from body fluids outside the body prior to introduction of the decontaminated body fluids into the body of a patient, said method comprising:

admixing an effective, non-toxic amount of a photoactive compound with the body fluid to product a resulting body fluid, the photoactive compound having an affinity to be selectively bound to the contaminants;

passing the resulting fluid under flow conditions through a cell assembly having a predetermined flow path; and

irradiating the resulting fluid in the cell assembly as same passes through the flow path with an effective level of radiation in the region of the visible spectrum, with a wavelength range above 400 nm to about 1000 nm, for an effective period of time such that the radiation penetrates the resulting fluid and exposes the photoactive-compound-bound contaminants to the radiation so as to eradicate such contaminants while maintaining the viability of said body fluids to produce viable decontaminated body fluids.

2. The eradicating method as recited in claim 1 further comprising the step of selecting a body fluid from the group consisting of whole blood, blood plasma, serum, and fluids from plasmapheresis.

3. The eradicating method as recited in claim 1 further comprising the step of selecting body fluids which contain an envelope-containing virus.

4. The eradicating method as recited in claim 1 further comprising the step of selecting body fluids which contain pathogenic biological contaminants comprising a malarial parasite.

5. A method of extracorporeal treatment of the blood of a patient infected with infectious pathogenic biological contaminants said method comprising:

removing blood from the body of a patient infected with infectious pathogenic biological contaminants;

adding to said blood, before the removal of the blood, an effective, non-toxic amount of photoactive compound having an affinity to be selectively bound to the infectious contaminants;

passing said treated blood through a cell assembly having a predetermined flow path;

irradiating said contaminated blood admixed with photoactive compound in the cell assembly as same passes through the flow path with an effective level of radiation in the region of visible spectrum, with a wavelength range 400 nm to about 1000 nm, for an effective period of time such that the radiation penetrates the blood and exposes the photoactive-compound-bound infectious contaminants to the radiation so as to eradicate such infectious contaminants while maintaining the viability of components in said blood to produce a viable and decontaminated blood; and

returning said viable and decontaminated blood to the patient'"'"'s body.

6. The method of claim 5 wherein the level of radiation is produced by a light source having a wavelength of from about 600 to about 1000 nm.

7. The eradicating method as recited in claim 5 further comprising the step of selecting a blood which contains pathogenic biological contaminants comprising an envelope-containing virus.

8. The eradicating method as recited in claim 5 further comprising the step of selecting the Retroviridae comprising a human immunodeficiency virus.

9. The eradicating method as recited in claim 5 further comprising the step of selecting the pathogenic biological contaminants comprising a malarial parasite.

10. The eradicating method as recited in claim 5 further comprising the step of selecting a blood which contains pathogenic biological contaminants comprising a trypanosomal parasite.

11. A method for extracorporeal treatment of the blood of a patient infected with infectious pathogenic biological contaminants, said method comprising:

removing blood from the body of a patient infected with infectious pathogenic foreign biological contaminants;

adding to said blood, after the removal of the blood, an effective non-toxic amount of photoactive compound having an affinity to be selectively bound to the infectious contaminants;

passing said treated blood through a cell assembly having a predetermined flow path;

irradiating said contaminated blood admixed with photoactive compound in the cell assembly as the same passes through the flow path with an effective level of radiation in the region of visible spectrum, with a wavelength range above 400 nm to about 1000 nm, for an effective period of time such that the radiation penetrates the blood and exposes the photoactive-compound-bound infectious contaminants to the radiation so as to eradicate such infectious contaminants while maintaining the viability of compounds in said blood to produce a viable and decontaminated blood; and

returning said viable and decontaminated blood to the patient'"'"'s body.