Liquid transfer device for an analysis unit

• US 5,304,347 A
• Filed: 02/05/1993
• Issued: 04/19/1994
• Est. Priority Date: 02/08/1992
• Status: Expired due to Term

• Current Assignee: Komori Corporation, Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Sigl, Andrea, Mann, Karl-Heinz, Geissler, Leonhard
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/014,271
• Time in Patent Office: 438 Days
• Field of Search: 422/50, 422/63, 422/65, 422/66, 422/67, 422/102, 422/64, 422/100, 73/61.63, 137/88, 137/93
• US Class Current: 422/67
• CPC Class Codes: G01F 23/263 by measuring variations in capacitance of capacitors
• CPC Class Codes: G01F 23/266 measuring circuits therefor
• CPC Class Codes: G01F 23/268 mounting arrangements of probes
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 35/1009 Characterised by arrangements for controlling the aspiration or dispense of liquids

Abstract

Liquid transfer device for an analysis unit with a capacitive liquid level detector (10) for detecting the dipping of a liquid transfer needle (5) into an analysis liquid (4). More reliable operation, in particular a better ratio between useful signal and noise signals, is achieved by the fact that the liquid transfer needle (5) is part of a coaxial electrode arrangement (11). The coaxial electrode arrangement includes, in addition to the liquid transfer needle (5), at least one coaxial electrode (12, 13) surrounding the needle and insulating from it and a detection circuit (17). This detection circuit detects a change in capacitance between the electrodes (5, 13) of the liquid level detector (10), and includes an isolation amplifier circuit (23) to whose input (23a) and output (23b) two adjacent electrodes (5, 12) of the coaxial electrode arrangement (11) are connected as signal electrode and compensating electrode. As a result of this structure, no voltage difference occurs between the signal electrode and the compensating electrode.

Claims

1. A liquid transfer device for an analysis unit, the liquid transfer device comprising:

a liquid transfer needle having a tip;

a capacitive liquid level detector for detecting a dipping of the tip of the liquid transfer needle into an analysis liquid, the analysis liquid being contained in a vessel, said capacitive liquid level detector comprising

a coaxial electrode arrangement including said liquid transfer needle and a first coaxial electrode coaxially surrounding said liquid transfer needle with the exception of the tip which projects therefrom, said first coaxial electrode being electrically insulated from said liquid transfer needle, wherein said liquid transfer needle and said first coaxial electrode of said coaxial electrode arrangement are coupled to a detection circuit comprising isolation amplifier circuit means for producing an output signal which corresponds to the input signal in phase and level, wherein an output side internal resistance of said isolation amplifier circuit means is lower than an input resistance thereof;

said liquid level detector further comprising

an a.c. voltage source connected to an input of said isolation amplifier circuit means and one of said first coaxial electrode and said liquid transfer needle, said one of the liquid transfer needle and said coaxial electrode forming a signal electrode,

wherein the other of said liquid transfer needle and said first coaxial electrode is coupled to an output of said isolation amplifier circuit means and forms a compensating electrode.

2. A liquid transfer device according to claim 1, wherein said liquid transfer needle is connected to the input of said isolation amplifier circuit and said first coaxial electrode is connected to the output of the isolation amplifier circuit.

3. A liquid transfer device according to claim 1, wherein the coaxial electrode arrangement further comprises a second coaxial electrode which surrounds the signal electrode and the compensating electrode and is connected to a constant potential whereby it simultaneously forms a screening electrode and a counterelectrode of said capacitive liquid level detector.

4. A liquid transfer device according to claim 1 wherein the signal electrode is coupled to the a.c. voltage source through a load resistor.

5. A liquid transfer device according to claim 4, wherein said load resistor has a value which corresponds to an alternating current resistance of a useful capacitance at a selected frequency of the a.c. voltage source.

6. A liquid transfer device according to claim 5, wherein said detection circuit comprises a second isolation amplifier circuit means coupled to said a.c. voltage source, said detection circuit further comprising a differential amplifier connected to outputs of said first and second isolation amplifier circuit means, wherein said first isolation amplifier circuit means is identical to said second isolation amplifier circuit means.

7. A liquid transfer device according to claim 6, wherein said first and second isolation amplifier circuit means are integrated monolithically.

8. A liquid transfer device according to claim 1 further comprising electrical conductors connecting the coaxial electrode arrangement to the detection circuit, said electrical conductors comprising screened coaxial lines.

9. A liquid transfer device according to claim 8, further comprising hose means connected to said liquid transfer needle, said hose means for intaking and ejecting a liquid medium, said hose means including electrical screening.

10. A liquid transfer device for an analysis unit, the liquid transfer device comprising:

a liquid transfer needle having a tip;

a capacitive liquid level detector for detecting a dipping of the tip of the liquid transfer needle into an analysis liquid, the analysis liquid being contained in a vessel, said capacitive liquid level detector comprising

a coaxial electrode arrangement including said liquid transfer needle and at least two coaxial electrodes coaxially surrounding said liquid transfer needle with the exception of the tip which projects therefrom, said at least two coaxial electrodes being electrically insulated from said liquid transfer needle and from each other, wherein liquid transfer needle and said coaxial electrodes of said coaxial electrode arrangement are coupled to a detection circuit comprising isolation amplifier circuit means for producing an output signal which corresponds to the input signal in phase and level, wherein an output side internal resistance of said isolation amplifier circuit means is lower than an input resistance thereof;

said liquid level detector further comprising

an a.c. voltage source connected to an input of said isolation amplifier circuit means and a first one of said at least two coaxial electrodes, said first one of said at least two coaxial electrodes forming a signal electrode,

wherein the other of said at least two coaxial electrodes is coupled to an output of said isolation amplifier circuit means and forms a compensating electrode.

11. A liquid transfer device according to claim 10, wherein the signal electrode is coupled to the a.c. voltage source through a high-value load resistor.

12. A liquid transfer device according to claim 11, wherein said load resistor has a value which corresponds to an alternating current resistance of a useful capacitance at a selected frequency of the a.c. voltage source.

13. A liquid transfer device according to claim 10, wherein said detection circuit comprises a second isolation amplifier circuit means coupled to said a.c. power source, said detection circuit further comprising a differential amplifier connected to outputs of said first and second isolation amplifier circuit means, wherein said first isolation amplifier circuit means is identical to said second isolation amplifier circuit means.

14. A liquid transfer device according to claim 13, wherein said first and second isolation amplifier circuit means are integrated monolithically.

15. A liquid transfer device according to claim 10, further comprising electrical conductors connecting the coaxial electrode arrangement to the detection circuit, said electrical conductors comprising screened coaxial lines.

16. A liquid transfer device according to claim 15, further comprising hose means connected to said liquid transfer needle, said hose means for intaking and ejecting a liquid medium, said hose means including electrical screening.

17. A liquid transfer device according to claim 10, wherein said coaxial electrode arrangement comprises at least three coaxial electrodes said signal electrode being arranged between two other electrodes of said coaxial electrode arrangement both of which are coupled to the output of said isolation amplifier circuit means and form compensating electrodes.

18. A liquid transfer device according to claim 17, wherein the signal electrode is coupled to the a.c. voltage source through a load resistor.

19. A liquid transfer device according to claim 18, wherein said load resistor has a value which corresponds to an alternating current resistance of a useful capacitance at a selected frequency of the a.c. voltage source.

20. A liquid transfer device according to claim 17, wherein said detection circuit comprises a second isolation amplifier circuit means coupled to said a.c. power source, said detection circuit further comprising a differential amplifier connected to outputs of said first and second isolation amplifier circuit means, wherein said first isolation amplifier circuit means is identical to said second isolation amplifier circuit means.

21. A liquid transfer device according to claim 20, wherein said first and second isolation amplifier circuit means are integrated monolithically.

22. A liquid transfer device according to claim 17, further comprising electrical conductors connecting the coaxial electrode arrangement to the detection circuit, said electrical conductors comprising screened coaxial lines.

23. A liquid transfer device according to claim 22, further comprising hose means connected to said liquid transfer needle, said hose means for intaking and ejecting a liquid medium, said hose means including electrical screening.

24. A method for detecting a level of liquid in a vessel of an analysis unit, said method comprising the steps of:

providing a liquid transfer device for an analysis unit, the liquid transfer device comprising a liquid transfer needle having a tip thereupon, and a capacitive liquid level detector for detecting a dipping of the tip of the liquid transfer needle into an analysis liquid, the analysis liquid being contained in a vessel, wherein the capacitive liquid level detector comprises a coaxial electrode arrangement including the liquid transfer needle and a first coaxial electrode coaxially surrounding the liquid transfer needle with the exception of the tip which projects therefrom, said first coaxial electrode being electrically insulated from said liquid transfer needle, wherein said liquid transfer needle and said first coaxial electrode of said coaxial electrode arrangement are coupled to a detection circuit comprising isolation amplifier circuit means for producing an output signal which corresponds to the input signal in phase and level, wherein an output side internal resistance of the isolation amplifier circuit means is lower than an input resistance thereof, with the liquid level detector further comprising an a.c. voltage source connected to an input of the isolation amplifier circuit means and one of the first coaxial electrode and the liquid transfer needle, with the one of the liquid transfer needle and the coaxial electrode forming a signal electrode, wherein the other of the liquid transfer needle and the first coaxial electrode is coupled to an output of the isolation amplifier circuit means, and forms a compensating electrode;

said method further comprising the steps of

applying an input signal to said detection circuit and one of said liquid transfer needle and said first coaxial electrode;

applying an output signal of said detection circuit to another of said liquid transfer needle and said first coaxial electrode, thereby forming a stray field therebetween, said stray field forming a useful capacitance;

actively compensating other capacitance of the coaxial electrode arrangement; and

measuring a change in voltage at an input of the isolation amplifier circuit means, said change in voltage being measured as a useful signal which is indicative of contacting of said liquid transfer needle to said analysis liquid.