Composition of matter comprising an imprinted matrix exhibiting selective binding interactions through chelated metals

• US 5,310,648 A
• Filed: 02/01/1991
• Issued: 05/10/1994
• Est. Priority Date: 02/01/1991
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Dhal, Pradeep, Plunkett, Sean, Shnek, Deborah, Arnold, Frances H.
• Primary Examiner(s): Hill, Jr., Robert J.
• Assistant Examiner(s): WANG, GIAN
• Application Number:
  US07/649,470
• Time in Patent Office: 1,194 Days
• Field of Search: 435/2, 435/4, 435/5, 435/34, 435/7.21, 435/7.23, 435/240.2, 435/235, 530/350, 530/395
• US Class Current: 435/5
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/3265 with an organic functional group containing a metal, e.g. a metal affinity ligand
• CPC Class Codes: C07C 229/16 to carbon atoms of hydrocarbon radicals substituted by amino or carboxyl groups, e.g. ethylenediamine-tetra-acetic acid, iminodiacetic acids
• CPC Class Codes: C07C 233/38 having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton
• CPC Class Codes: C07F 9/10 Phosphatides, e.g. lecithin
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: G01N 33/545 Synthetic resin

Abstract

The invention provides an imprinted matrix which exhibits selective binding interactions through metal chelates with a predetermined molecule or biological particle. Also provided is a preformed, fluid-imprinted matrix having sufficient rigidity to maintain selective binding interaction with a predetermined molecule or biological particle through interactive moieties. Methods for producing such matrices are additionally provided.

Claims

1. A composition of matter which selectively binds to one or more functional groups present at spaced locations on the surface of a template molecule, said composition comprising:

an imprinted matrix comprising a polymer structure which is spatially matched to the surface of said template molecule;

one or more interactive moieties located on said polymer at spaced locations which are complementary to said functional groups of said template molecule, said interactive moieties comprising chelated metals wherein said chelated metals are located so as to undergo selective binding interactions with said functional groups to thereby provide selective binding of said composition of matter to said template molecule.

2. A composition of matter according to claim 1 wherein said imprinted matrix is spatially matched to the source of a template molecule comprising a macromolecule, said macromolecule including functional groups and wherein said interactive moieties are located at spaced locations on said imprinted matrix which are complementary to the functional groups present on said macromolecule.

3. A composition of matter according to claim 1 wherein said imprinted matrix is spatially matched to the surface of a template molecule comprising a cell or virus, said cell or virus including functional groups and wherein said interactive moieties are located at spaced locations on said imprinted matrix which are complementary to the functional groups present on said macromolecule.

4. A composition of matter according to claim 1 wherein said chelated metals are selected from the group consisting of Pt, Ru, Cu, Fe, Ni, Zn, Co, Cd and Ca.

5. A composition of matter according to claim 1 wherein said polymer structure is made from N-(4-vinyl)-benzyl iminodiacetic acid monomers.

6. A composition of matter according to claim 1 wherein said imprinted matrix is a lipid membrane.

7. A composition of matter according to claim 6 wherein said lipid membrane comprises the polymerizable monomer (bis 1,2-(2,4-octadecadienoyl)-sn-glycerol-3-(propyl) iminodiacetic acid.

8. A composition of matter according to claim 6 wherein said lipid membrane comprises the polymerizable monomer rac 1,2-(2,4-octadecadienoyl)-sn-glycerol-3-phosphoryl(propyl) iminodiacetic acid.

9. A composition of matter according to claim 6 wherein said lipid membrane comprises the polymerizable monomer rac 1,2-bis (2,4-octadecadienoyl)-sn-glycerol-3-phosphoric acid.