Method of measuring an analyte by measuring electrical resistance of a polymer film reacting with the analyte

• US 5,312,762 A
• Filed: 09/13/1991
• Issued: 05/17/1994
• Est. Priority Date: 03/13/1989
• Status: Expired due to Fees

• Current Assignee: Anthony Guiseppi-Elie
• Original Assignee: Anthony Guiseppi-Elie
• Inventors: Guiseppi-Elie, Anthony
• Primary Examiner(s): Naff, David M.
• Application Number:
  US07/760,450
• Time in Patent Office: 977 Days
• Field of Search: 435/4, 435/14, 435/25, 435/174, 435/177, 435/180, 435/181, 435/182, 435/817, 204/403, 436/149, 436/806
• US Class Current: 205/778
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: C08F 8/00 Chemical modification by after-treatment graft polymers, block polymers, crosslinking with unsaturated monomers or with polymers C08F251/00 - C08F299/00; of conjugated diene rubbers C08C
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

The change in electrical resistance of an electroactive polymer such as polypyrrole, polyaniline or polythiophene when it reacts with an analyte is used to measure the analyte. The open circuit electrical potential of an electroactive polymer film is measured and an electrical potential is applied to the polymer film relative to a reference electrode to oxidize or reduce the polymer film to provide an initial reduced or oxidized state. Then the electrical resistance of the polymer film is measured in the absence of an analyte and the electrical resistance is again measured while the polymer film reacts with the analyte. The analyte concentration is determined from the rte and total amount of electrical resistance change. The electrical resistance is preferably measured by applying a to the polymer film a discontinuous non-perturbating voltage pulse, removing the voltage for a period, making a measurement of open circuit potential and applying a subsequent discontinuous nonperturbating voltage pulse relative to the open-circuit potential. In measuring glucose as the analyte, reaction of glucose with glucose oxidase contained by the polymer film produces hydrogen peroxide which oxidizes the polymer film and makes it more conductive.

Claims

1. An analytical method for measuring an analyte that uses the interrogration of chemoresistive sensor responses from electroactive polymer films, said method using an electroactive polymer sensor device and comprising the following steps:

(a) measuring open circuit electrical potential an electroactive polymer film of said device;

(b) applying a potentiostatic electrical potential relative to a reference electrode to said polymer film which oxidizes or reduces said polymer film to provide an initial reduced or oxidized state, and thereby provides said polymer film with an initial electrical resistance;

(c) measuring the electrical resistance of the polymer film resulting from (b) by applying to the polymer film a non-perturbating voltage pulse, removing the voltage pulse from the polymer film for a period following the pulse duration, making a potentiometric measurement of the open circuit potential of the polymer film and applying to the polymer film a subsequent non-perturbating voltage pulse relative to the measured open circuit potential before said polymer film reacts with an analyte to which it has been made specific;

(d) reacting said polymer film with an analyte, to which it has been made specific whereby the reaction causes a change in electrical resistance of said polymer film;

(e) measuring the electrical resistance of the polymer film resulting from (d) by applying to the polymer film a non-perturbating voltage pulse, removing the voltage pulse from the polymer film for a period following the pulse duration, making a potentiometeric measurement of the open circuit potential of the polymer film and applying to the polymer film a subsequent non-perturbating voltage pulse relative to the emasured open circuit potential while said polymer film reacts with an analyte to which it has been made specific; and

(f) determining the rate of change of the electrical resistance and total amount of change of the electrical resistance from which the concentration of the analyte is determined.

2. The method of claim 1 wherein said electrical resistance measurement of the polymer film of said device is made by the following steps repeated in sequence;

(a) applying to the polymer film a non-perturbating voltage pulse in the range of plus or minus 5 to 25 millivolts for a specified pulse duration of between about 1 and 100 milliseconds;

(b) removing the voltage from the polymer film for a period following the pulse duration, the duration of said period being about 10 times the duration of the applied pulse;

(c) making a potentiometric measurement of the open circuit potential of the polymer film; and

(d) applying to the polymer film a subsequent non-perturbating voltage pulse relative to the measured open-circuit potential.

3. The method of claim 2 wherein said steps (a)-(d) are repeated.