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Preparation of novel immobilized biocatalysts

  • US 5,314,814 A
  • Filed: 02/03/1992
  • Issued: 05/24/1994
  • Est. Priority Date: 11/15/1985
  • Status: Expired due to Term
First Claim
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1. A method of preparing an immobilized water-soluble biocatalyst comprising living cells in particulate form consisting essentially of the steps of:

  • (a) suspending the living cells in an aqueous solution of a gelling agent,(b) combining the mixture so obtained with an organic liquid poorly miscible or immiscible in water to form a suspension in the organic liquid of aqueous particles comprising the living cells and gelling agent,(c) treating the suspension to gel the gelling agent in the particles,(d) treating the particles obtained in step (c) with a bi- or polyfunctional cross-linking agent to covalently crosslink the gelling agent in the particles, and(e) removing at least part of the water from the particles obtained in step (d) to obtain particles with a dry matter content of at least 15% by weight.

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