Synthesis of chain chemical compounds

• US 5,318,679 A
• Filed: 01/25/1993
• Issued: 06/07/1994
• Est. Priority Date: 08/07/1991
• Status: Expired due to Term

• Current Assignee: H & N Instruments Incorporated
• Original Assignee: H & N Instruments Incorporated
• Inventors: Nishioka, Gary M.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): DELACROIX MUIRHEI, CYBILLE
• Application Number:
  US08/008,131
• Time in Patent Office: 498 Days
• Field of Search: 204/157.61, 204/157.68, 372/15, 372/25, 436/518
• US Class Current: 204/157.68
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00436 Maskless processes
• CPC Class Codes: B01J 2219/00441 using lasers
• CPC Class Codes: B01J 2219/00475 Sheets
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00686 Automatic
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07C 231/02 : from carboxylic acids or from esters, anhydrides, or halides thereof by reaction with ammonia or amines
• CPC Class Codes: C07C 237/12 : having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by carboxyl groups peptides C07K
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

The present invention is an improved method of making sequenced chemical chain compounds through the application of laser copy technology to UV light directed spatially addressed parallel chemical synthesis and is particularly useful in the production of numerous proteins of varying amino acid sequences on a restricted solid support. Chemical groups, such as amines, are attached to the surface of a solid sup- port, such as flat glass, and subjected to chemical reaction disposed to link chemical units to the attached groups that are protected from further reaction by photolabile groups. Such solid support and photolabile protected units are then selectively deprotected by irradiation with a UV laser beam. The support and attached units are then subjected to chemical reaction to link chemical units to the selectively deprotected units. By repeated such treatment it is possible to produce unlimited combinations of chain chemical units in less time and at greater efficiency than has previously been possible.

Claims

1. In the process of making chemical compounds wherein photoprotected chemical units attached to the surface of a support are selectively irradiated to deprotect preselected areas of said surface so that the surface of said support may be subjected to chemical reaction disposed to attach chemical units to the deprotected units, the improvement in combination therewith of irradiating said surface with the laser beam of a laser printer, said laser having a wave length in the ultraviolet range.

2. The process of claim 1 wherein the chemical units attached to the deprotected units are photoprotected for subsequent irradiation with said laser beam.

3. The process of claim 2 wherein said units are subsequently irradiated with said laser beam and subjected to chemical reaction disposed to attach a chemical unit to the deprotected units.

4. The process of claim 3 wherein the steps of photo-protection of said units, irradiation and chemical reaction to attach a chemical unit to the deprotected units are repeated to achieve multichain chemical compounds.

5. The process of claim 1 wherein said laser beam is caused to scan said surface so as to effect parallel lines of deprotected areas.

6. The process of claim 5 wherein said laser beam is caused to pulsate on and off so as to effect individual restricted areas of deprotection.

7. The process of claim 5 wherein said support is rotated subsequent to such scan from 0 to 90 degrees to cause said lines to intersect.

8. The process of claim 1 wherein said laser beam wavelengths are above 3200 Angstroms.

9. The process of claim 1 wherein said laser beam printer comprises a laser beam directed onto the surface of a multisurfaced rotating mirror positioned so that each mirror surface reflects said beam onto said surface to scan said surface.

10. The process of claim 2 wherein the chemical units attached to said support are amines and the chemical units attached to the deprotected areas are amino acids.

11. The process of claim 3 wherein the chemical units attached to said support are amines and the chemical units subsequently attached to deprotected units are amino acids so that the linkage between the units are peptide linkages.

12. The process of claim 4 wherein the chemical units attached to said support are amines and the chemical units subsequently attached to deprotected units are amino acids so that the linkage between the units are peptide linkages.