Electrode and method for the detection of hydrogen peroxide
First Claim
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1. An electrode for the detection of hydrogen peroxide comprising:
- an electrode having a testing surface; and
a transducing film substantially covering the testing surface of the electrode, the film comprising a cross-linked redox polymer network, the network comprising a redox compound having multiple redox centers and a peroxidase having redox centers wherein the redox centers of the peroxidase are electrically connected, in the absence of diffusional electron shuttles, to the electrode.
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Abstract
An amperometric biosensor and method for the detection of hydrogen peroxide, NADH, or NADPH with high sensitivity includes an electrode having on its testing surface a three-dimensional redox polymer network in which peroxidase is immobilized.
1306 Citations
17 Claims
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1. An electrode for the detection of hydrogen peroxide comprising:
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an electrode having a testing surface; and a transducing film substantially covering the testing surface of the electrode, the film comprising a cross-linked redox polymer network, the network comprising a redox compound having multiple redox centers and a peroxidase having redox centers wherein the redox centers of the peroxidase are electrically connected, in the absence of diffusional electron shuttles, to the electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for analyzing the presence of hydrogen peroxide in a test sample comprising the steps of:
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contacting a test sample with an electrode having a test surface substantially covered with a transducing film comprising a cross-linked redox polymer network having multiple redox centers and peroxidase immobilized in the network; generating electrons at the electrode; transferring electrons from the electrode through the cross-linked polymer network to the peroxidase; electroreducing hydrogen peroxide at the peroxidase; generating an electrical current by electroreduction of hydrogen peroxide within the cross-linked redox polymer network; and measuring the generated current. - View Dependent Claims (14, 15)
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16. A method for analyzing the presence of NADPH or NADH in a test sample comprising the steps of:
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reacting NADPH or NADH with dissolved molecular oxygen in the presence of a quinoid in the test sample; contacting the reacted test solution with an electrode having a testing surface substantially covered with a transducing film comprising a cross-linked redox polymer network having multiple redox centers and peroxidase immobilized in the network; generating electrons at the electrode; transferring electrons from the electrode through the cross-linked polymer network to the peroxidase; electroreducing hydrogen peroxide at the immobilized peroxidase; generating an electrical current by the transfer of electrons and electroreduction of hydrogen peroxide; and quantifying the generated current. - View Dependent Claims (17)
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Specification