Immunoassay using magnetic particle
First Claim
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1. An immunoassay method comprising the steps of:
- (a) reacting a sample containing an antigen with an antibody which specifically binds to said antigen, so as to bind said antigen to said antibody, wherein said antibody is immobilized on a 0.2 to 3.0 μ
m magnetic particle comprising a core and a coating layer on the surface of said core, wherein said core comprises an organic polymer and said coating layer comprises a metal oxide formed by adding ferrous ions or ferrous ions and an ion selected from the group consisting of zinc, cobalt, nickel, manganese, copper, vanadium, antimony, lithium, molybdenum, titanium, rubidium, aluminum, silicon, chromium, tin, calcium, cadmium, indium ions and mixtures thereof to deoxidized water in which cores of organic polymer are suspended to form a resulting solution, and subsequently adding an oxidizing agent solution to the resulting solution to provide a ferrite coating on said core;
(b) measuring an amount of bound antigen; and
(c) correlating the amount of bound antigen obtained in step (b) with an amount of antigen in the sample.
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Abstract
An immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, The core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer. An antigen or an antibody is bound onto the surface of the coating layer and the particle has a particle size of 0.2 to 3 μm.
59 Citations
22 Claims
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1. An immunoassay method comprising the steps of:
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(a) reacting a sample containing an antigen with an antibody which specifically binds to said antigen, so as to bind said antigen to said antibody, wherein said antibody is immobilized on a 0.2 to 3.0 μ
m magnetic particle comprising a core and a coating layer on the surface of said core, wherein said core comprises an organic polymer and said coating layer comprises a metal oxide formed by adding ferrous ions or ferrous ions and an ion selected from the group consisting of zinc, cobalt, nickel, manganese, copper, vanadium, antimony, lithium, molybdenum, titanium, rubidium, aluminum, silicon, chromium, tin, calcium, cadmium, indium ions and mixtures thereof to deoxidized water in which cores of organic polymer are suspended to form a resulting solution, and subsequently adding an oxidizing agent solution to the resulting solution to provide a ferrite coating on said core;(b) measuring an amount of bound antigen; and (c) correlating the amount of bound antigen obtained in step (b) with an amount of antigen in the sample. - View Dependent Claims (3, 5, 7, 9, 11, 13, 15, 17, 19, 21)
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2. An immunoassay method comprising the steps of:
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(a) reacting a sample containing an antibody with an antigen which specifically binds to said antibody, so as to bind said antibody to said antigen, wherein said antigen is immobilized on a 0.2 to 3.0 μ
m magnetic particle comprising a core and a coating layer on the surface of said core, wherein said core comprises an organic polymer and said coating layer comprises a metal oxide formed by adding ferrous ions or ferrous ions and an ion selected from the group consisting of zinc, cobalt, nickel, manganese, copper, vanadium, antimony, lithium, molybdenum, titanium, rubidium, aluminum, silicon, chromium, tin, calcium, cadmium, indium ions and mixtures thereof to deoxidized water in which cores of organic polymer are suspended to form a resulting solution, and subsequently adding an oxidizing agent solution to the resulting solution to provide a ferrite coating on said core;(b) measuring an amount of bound antibody; and (c) correlating the amount of bound antibody obtained in step (b) with an amount of antibody in the sample. - View Dependent Claims (4, 6, 8, 10, 12, 14, 16, 18, 20, 22)
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Specification