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Bioreactor for the perfusion culture of cells

  • US 5,320,963 A
  • Filed: 11/25/1992
  • Issued: 06/14/1994
  • Est. Priority Date: 11/25/1992
  • Status: Expired due to Fees
First Claim
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1. An apparatus for perfusion cell culture comprising:

  • a cell culture tank having a suspension cell culture zone, a cell settling zone, an opening at the top of the tank for supplying a culture medium to said cell culture zone and an opening for discharging a spent culture medium from said settling zone,the settling zone being disposed annularly in the upper portion of the tank co-extensively, in a vertical direction, with a part of the cell culture zone,a partition disposed between said zones to enable said zones to communicate with each other only below said partition,a centrally disposed substantially cylindrical draft tube extending substantially vertically along at least a part of said cell culture zone to define a passage between said draft tube and the partition,wherein the tank has upwardly diverging side walls at least in the upper portion of said tank which houses said settling zone, the partition has upwardly diverging walls substantially parallel to the side walls of the tank thereby defining a generally inversely-frustoconical shape of the settling zone, andthe settling zone comprises means defining a plurality of angularly disposed passageways of upwardly increasing cross-sectional area, said passageways being substantially parallel to the walls of the tank in the settling zone and to the wall of the partition.

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