Aqueous dispersions of zein and preparation thereof

• US 5,324,351 A
• Filed: 08/13/1992
• Issued: 06/28/1994
• Est. Priority Date: 08/13/1992
• Status: Expired due to Fees

• Current Assignee: Euroceltique SA
• Original Assignee: Euroceltique SA
• Inventors: Bodmeier, Roland, Oshlack, Benjamin, Chasin, Mark, McGinity, James
• Primary Examiner(s): BRUNSMAN, DAVID M
• Application Number:
  US07/930,107
• Time in Patent Office: 684 Days
• Field of Search: 106/153, 530/373
• US Class Current: 106/156.3
• CPC Class Codes: A23J 3/18 from wheat
• CPC Class Codes: A23P 20/10 Coating with edible coatings, e.g. with oils or fats
• CPC Class Codes: A61K 9/2873 Proteins, e.g. gelatin
• CPC Class Codes: A61K 9/5052 Proteins, e.g. albumin
• CPC Class Codes: A61K 9/5078 with drug-free core
• CPC Class Codes: C08J 2389/00 Characterised by the use of proteins; Derivatives thereof
• CPC Class Codes: C08J 3/07 from polymer solutions
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C09D 189/00 Coating compositions based on proteins; Coating compositions based on derivatives thereof foodstuff preparations A23J3/00

Abstract

Aqueous dispersions of zein which may be used in the coating of pharmaceutical, animal, health, or food products in an inorganic solvent-free environment are disclosed, as well as methods for preparing the same.

Claims

1. A method for preparing an aqueous dispersion of zein, comprising

preparing a solvent mixture comprising water and from about 60 to about 90 percent of an organic solvent selected from the group consisting of ethanol, acetone, and mixtures thereof,

adding a material consisting essentially of zein to said solvent mixture in such a proportion to said solvent mixture that the zein dissolves in the solvent mixture,

precipitating the zein by pouring said solution of zein as a stream into an aqueous phase under continuous mixing such that the zein precipitates as fine particles in a colloidal dispersion, and

removing the organic solvent to obtain an aqueous dispersion comprising from about 0.1 to about 10 percent w/v of zein.

2. The method of claim 1, wherein said solvent mixture comprises ethanol:

water in a ratio of about 8;

2 v/v.

3. The method of claim 1, wherein said solvent mixture comprises ethanol:

water in a ratio of about 6;

4 v/v.

4. The method of claim 1, wherein said solvent mixture comprises acetone:

water in a ratio of about 8;

2 v/v.

5. The method of claim 1, wherein said solvent mixture comprises acetone:

water in a ratio of about 6;

4 v/v.

6. The method of claim 1, wherein the pH of the resulting aqueous zein dispersion is from about 4 to about 6.

7. The method of claim 1, further comprising adding an effective amount of a plasticizer to said aqueous dispersion of zein to provide a flexible film when said aqueous dispersion is applied to a substrate.

8. The method of claim 1, further comprising adding a solubilizing agent to said aqueous dispersion of zein.

9. The method of claim 1, further comprising adding a suitable buffer system to adjust the pH of said aqueous dispersion of zein to above about pH 9.

10. The zein powder produced by the method of claim 9.

11. The method of claim 1, further comprising adding an ammonium carbonate-ammonia buffer to said aqueous dispersion of zein to adjust the pH to above about pH 9.

12. A method for preparing a redispersible zein powder, comprising

preparing a solvent mixture comprising water and from about 60 to about 90 percent of an organic solvent selected from the group consisting of ethanol, acetone, and mixtures thereof,

adding a material consisting essentially of zein to said solvent mixture in such a proportion to said solvent mixture that the zein dissolves in the solvent mixture,

precipitating the zein by pouring said solution of zein as a stream into an aqueous phase under continuous mixing such that the zein precipitates as fine particles in a colloidal dispersion and removing the organic solvent to obtain an aqueous dispersion comprising from about 0.1 to about 10 percent w/v of zein, and

drying the resultant aqueous dispersion to obtain a zein powder which is redispersible in aqueous solutions.

13. The method of claim 12, wherein said aqueous dispersion is spray-dried to obtain a redispersible zein powder having a particle size ranging from about 0.1 to about 10 μ

m.

14. The method of claim 13, further comprising redispersing the zein powder in an aqueous solution prior to use in a coating process.

15. The zein powder produced by the method of claim 12.

16. A zein powder having a particle size ranging from about 0.1 to about 10 μ

m which is redispersible in an aqueous solution to provide an aqueous dispersion of zein wherein said zein is obtained by the process of claim 12.

17. An aqueous dispersion of zein produced by adding a material consisting essentially of zein to a solvent mixture comprising water and from about 60 to about 90 percent of an organic solvent selected from the group consisting of ethanol, acetone, and mixtures thereof, in such a proportion that the zein dissolves in the solvent mixture, and pouring said solution of zein as a stream into an aqueous phase under continuous mixing such that the zein precipitates as fine particles in a colloidal dispersion, the organic solvent thereafter being removed to obtain an aqueous dispersion comprising from about 0.1 to about 10 percent w/v of zein.

18. The aqueous dispersion of claim 17 comprising zein and a buffer in an amount effective to adjust the pH of the aqueous dispersion to above about pH 9.

19. The aqueous dispersion of claim 18, wherein said buffer is selected from the group consisting of an ammonium carbonate-ammonia buffer, a citric acid-sodium phosphate buffer, and a boric acid-potassium chloride-sodium hydroxide buffer.

20. The aqueous dispersion of claim 17, which has a pH from about 3 to about 7.

21. The aqueous disperion of claim 17, which comprises from about 5 to about 10 percent zein w/v, and having a pH from about 4 to about 6.

22. The aqueous dispersion of claim 17, wherein the particle size of the zein in said aqueous dispersion is from about 0.01 to about 2.0 μ

m.

23. The aqueous dispersion of claim 17, wherein the average particle size of the zein in said aqueous dispersion is from about 100 to about 300 nanometers.

24. The aqueous dispersion of claim 17, wherein the pH of the aqueous dispersion is from about 4.5 to about 5.5.