Enzyme-containing granules coated with hydrolyzed polyvinyl alcohol or copolymer thereof

• US 5,324,649 A
• Filed: 10/07/1992
• Issued: 06/28/1994
• Est. Priority Date: 10/07/1991
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Wendt, Daniel J., Arnold, Raymond E., Mansikkamaki, Aino, Becker, Nathaniel T., Boston, Matthew G., Simpson, Curran M.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US07/957,973
• Time in Patent Office: 629 Days
• Field of Search: 435/182, 435/180, 435/187, 435/175, 435/176, 435/177, 435/180, 435/182, 435/264, 252/174.12, 252/DIG. 12
• US Class Current: 435/187
• CPC Class Codes: C11D 17/0039 Coated compositions or coated components in the compositions, (micro)capsules
• CPC Class Codes: C11D 3/3753 Polyvinylalcohol; Ethers or esters thereof
• CPC Class Codes: C11D 3/3776 Heterocyclic compounds, e.g. lactam
• CPC Class Codes: C11D 3/38672 Granulated or coated enzymes
• CPC Class Codes: C12N 11/02 Enzymes or microbial cells immobilised on or in an organic carrier
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12N 11/14 Enzymes or microbial cells immobilised on or in an inorganic carrier
• CPC Class Codes: C12N 9/98 Preparation of granular or free-flowing enzyme compositions C12N9/96 takes precedence

Abstract

A granular enzyme composition is produced, having reduced tendencies to form dust and leave residue, and exhibiting improved stability and delayed release characteristics. The granular composition comprises a core, an enzyme layer and an outer coating layer. The enzyme layer, and optionally the core and outer coating layer, contain a vinyl polymer. The vinyl polymer is preferably a hydrolyzed polyvinyl alcohol or copolymer thereof. The hydrolyzed polyvinyl alcohol has varying degrees of hydrolysis in the core, enzyme layer and outer coating layer. Also disclosed are methods for making such enzyme-containing granules, the methods having greatly reduced processing time.

Claims

1. An enzyme-containing granule comprising:

a) a non-enzyme containing core comprising a water soluble or water dispersible material coated with polyvinyl alcohol or copolymer thereof hydrolyzed about 70-90%;

an enzyme layer comprising a mixture of at least one enzyme and polyvinyl alcohol or copolymer thereof hydrolyzed about 98-99%; and

c) an outer coating layer comprising polyvinyl alcohol or copolymer thereof hydrolyzed about 70-90%.

2. A granule of claim 1 wherein the copolymer in steps a), b) or c) is polyvinyl alcohol-methylmethacrylate copolymer.

3. A granule of claim 1 wherein the polyvinyl alcohol or copolymer thereof in a) has a low viscosity.

4. A granule of claim 1 wherein the core comprises a nonpareil.

5. A granule of claim 4 wherein the polyvinyl alcohol or copolymer thereof has a low viscosity.

6. A granule of claim 1 wherein the core further comprises a plasticizer.

7. A granule of claim 1 wherein the enzyme layer comprises at least one enzyme selected from the group consisting of protease, amylase, lipase and cellulase.

8. A granule of claim 7 wherein the enzyme is a protease.

9. A granule of claim 8 wherein the protease is a subtilisin.

10. A granule of claim 7 wherein the enzyme is a cellulase or a component thereof.

11. A granule of claim 7 wherein the enzyme is a lipase.

12. A granule of claim 7 wherein the enzyme is a protease and a cellulase.

13. A granule of claim 1 wherein the polyvinyl alcohol or copolymer thereof in b) has a low viscosity.

14. A granule of claim 1 wherein the enzyme layer further comprises:

a) a plasticizer;

or

b) an anti-agglomeration agent.

15. A granule of claim 1 wherein the polyvinyl alcohol or copolymer thereof in c) has a low viscosity.

16. A granule of claim 1 wherein the outer coating layer c) further comprises a low residue pigment.

17. A granule of claim 16 wherein the pigment is titanium dioxide.

18. A granule of claim 1 wherein the outer coating layer c) further comprises a lubricant.

19. A granule of claim 18 wherein the lubricant is a nonionic or anionic surfactant.

20. A granule of claim 19 wherein the surfactant is a linear primary alcohol of a 9-15 carbon atom chain length alkane or alkene or an ethoxylate or ethoxysulfate derivative thereof.

21. A granule of claim 1 further comprising a scavenger layer.

22. A granule of claim 21 wherein the scavenger layer comprises ammonium sulfate.

23. An enzyme-containing granule comprising:

a) a non-enzyme containing nonpareil core;

b) an enzyme layer comprising at least one enzyme and polyvinyl alcohol hydrolyzed about 98-99% and having low viscosity; and

c) an outer coating layer comprising polyvinyl alcohol hydrolyzed about 70-90% and having low viscosity.

24. A granule of claim 23 further comprising a low residue pigment in the outer coating.

25. A granule of claim 24 further comprising a lubricant in the outer coating.

26. A granule of claim 25 wherein the lubricant is a lubricant is a nonionic or ionic surfactant.

27. A granule of claim 26 wherein the surfactant is a linear primary alcohol of 9-15 carbon atom chain length alkane or alkene or an ethoxylate or ethoxysulfate derivative thereof.

28. A granule of claim 23 further comprising a scavenger layer.

29. An enzyme-containing granule comprising:

a) a non-enzyme containing nonpareil core coated with polyvinyl alcohol hydrolyzed about 70-90% and having low viscosity;

b) an enzyme layer comprising at least one enzyme and polyvinyl alcohol hydrolyzed about 98-99% and having low viscosity; and

c) an outer coating layer comprising a polyvinyl alcohol hydrolyzed about 70-90% and having a low viscosity, a low residue pigment and a linear alkane or alkene primary alcohol of 9-15 carbon atom chain length, or an ethoxylate or ethoxysulfate derivative thereof.

30. A granule of claim 29 further comprising a scavenger layer.

31. A granule of claim 29 wherein the nonpareil core is further coated with a plasticizer.

32. A granule of claim 29 wherein the enzyme layer further comprises:

a) a plasticizer;

or

b) an anti-agglomeration agent.

33. A granule of claim 29 wherein the enzyme layer comprises at least one enzyme selected from the group consisting of protease, amylase, lipase and cellulase.

34. A method for making an enzyme-containing granule, said method comprising:

a) selecting a water soluble or dispersible core material;

b) coating the core of step a) with an enzyme layer comprising a mixture of at least one enzyme and polyvinyl alcohol or copolymer thereof hydrolyzed about 98-99%; and

c) coating the product of step b) with polyvinyl alcohol or copolymer thereof hydrolyzed about 70-90%;

alone or in combination with one or more pigments or lubricants.

35. A method of claim 34 comprising:

a) selecting as the core material a nonpareil core coated with polyvinyl alcohol hydrolyzed about 98-99% and having a low viscosity;

b) coating the coated core of step a) with at least one enzyme and polyvinyl alcohol hydrolyzed about 98-99% and having a low viscosity; and

c) coating the product of step b) with an integral mixture comprising;

1) polyvinyl alcohol hydrolyzed about 70-90% and having a low viscosity;

2) a low residue pigment; and

3) a lubricant.

36. A method of claim 35 wherein the lubricant of step c) is a nonionic or ionic surfactant.

37. A method of claim 36 wherein the surfactant is a linear alkane or alkene primary alcohol of 9-15 carbon atom chain length, or an ethoxylate or ethoxysulfate derivative thereof.

38. A method of claim 34 further comprising coating the product of step b) with a suitable chlorine scavenger prior to applying the coating of step c).