Fluorescent microparticles with controllable enhanced stokes shift
First Claim
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1. A fluorescent microparticle made by the process comprising:
- a) selecting a series of dyes comprising an initial donor dye with a desired excitation peak and a final acceptor dye with a desired emission peak, wherein the desired excitation peak and the desired emission peak are determined in a polymeric material, and wherein said polymeric material is made up of polymerizable monomers, and wherein each dye in the series has a spectral overlap sufficient to allow for significant energy transfer of excitation energy to the final acceptor dye, b) incorporating said series of dyes in a polymeric microparticle comprising said polymeric material, wherein said series of dyes are randomly incorporated into said polymeric microparticle.
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Abstract
The invention relates to microparticles incorporating a series of two or more fluorescent dyes having overlapping excitation and emission spectra allowing efficient energy transfer from the excitation wavelength of the first dye in the series, transfer through the dyes in the series and re-emitted as an optical signal at the emission wavelength of last dye in the series, resulting in a desired effective Stokes shift which is controlled through selection of appropriate dyes.
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Citations
15 Claims
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1. A fluorescent microparticle made by the process comprising:
- a) selecting a series of dyes comprising an initial donor dye with a desired excitation peak and a final acceptor dye with a desired emission peak, wherein the desired excitation peak and the desired emission peak are determined in a polymeric material, and wherein said polymeric material is made up of polymerizable monomers, and wherein each dye in the series has a spectral overlap sufficient to allow for significant energy transfer of excitation energy to the final acceptor dye, b) incorporating said series of dyes in a polymeric microparticle comprising said polymeric material, wherein said series of dyes are randomly incorporated into said polymeric microparticle.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A fluorescent microparticle comprising:
a series of fluorescent dyes having an initial donor dye with a desired excitation peak and an ultimate acceptor dye with a desired emission peak, wherein the desired excitation peak and the desired emission peak are determined in a polymeric material, wherein said polymeric material is made up of polymerizable monomers, and wherein each dye in the series has a spectral overlap sufficient to allow for significant energy transfer of excitation energy to the ultimate acceptor dye, wherein said series of dyes is randomly incorporated in a polymeric microparticle comprising said polymeric material such that the total dye concentration is less than about 10% w/w and the ratio of initial donor to ultimate acceptor is between about 1;
5 and about 1;
10.- View Dependent Claims (12, 13, 14, 15)
Specification